Hh signalling is essential for somatic stem cell maintenance in the Drosophila testis niche
Hh signalling is essential for somatic stem cell maintenance in the Drosophila testis niche
复制标题
DOI：
10.1242/dev.075242
复制
发表时间：
2012-08-01
期刊：
影响因子：
4.6
通讯作者：
Boekel, Christian
中科院分区：
文献类型：
--
作者：
Michel, Marcus;Kupinski, Adam P.;Boekel, Christian
在果蝇睾丸中，生殖系干细胞（GSC）和体细胞囊肿干细胞（CySC）排列在一组有丝分裂后的体细胞周围，称为枢纽，其产生多种生长因子，有助于调节两个干细胞池的微环境。在这里，我们表明，CySC，而不是GSC的维护需要刺猬（Hh）信号除了Jak/Stat途径激活。不能抑制Hh信号的CySC克隆因分化而丢失，而途径过度活化导致增殖增加。然而，与异位过表达Jak/Stat靶标的细胞不同，由过量Hh信号传导产生的额外细胞仍然局限于睾丸尖端并保留分化能力。有趣的是，Hh信号也控制着果蝇卵巢和哺乳动物睾丸中的体细胞群。因此，我们的观察结果可能指向更高程度的组织同源性之间的性腺体细胞成分的性别和门比以前认识到的。
In the Drosophila testis, germline stem cells (GSCs) and somatic cyst stem cells (CySCs) are arranged around a group of postmitotic somatic cells, termed the hub, which produce a variety of growth factors contributing to the niche microenvironment that regulates both stem cell pools. Here we show that CySC but not GSC maintenance requires Hedgehog (Hh) signalling in addition to Jak/Stat pathway activation. CySC clones unable to transduce the Hh signal are lost by differentiation, whereas pathway overactivation leads to an increase in proliferation. However, unlike cells ectopically overexpressing Jak/Stat targets, the additional cells generated by excessive Hh signalling remain confined to the testis tip and retain the ability to differentiate. Interestingly, Hh signalling also controls somatic cell populations in the fly ovary and the mammalian testis. Our observations might therefore point towards a higher degree of organisational homology between the somatic components of gonads across the sexes and phyla than previously appreciated.