Rpi-vnt1.1, a Tm-22 Homolog from Solanum venturii, Confers Resistance to Potato Late Blight
Rpi-vnt1.1, a Tm-22 Homolog from Solanum venturii, Confers Resistance to Potato Late Blight
复制标题
DOI：
10.1094/mpmi-22-5-0589
复制
发表时间：
2009-05-01
影响因子：
3.5
通讯作者：
Jones, Jonathan D. G.
中科院分区：
文献类型：
--
作者：
Foster, Simon J.;Park, Tae-Ho;Jones, Jonathan D. G.
尽管育种者做出了努力并广泛使用了杀菌剂控制措施，但晚疫病仍然是全世界马铃薯种植的主要威胁。将遗传抗性引入栽培马铃薯被认为是实现晚疫病持久抗性的重要方法。在这里，我们报告了 Rpi-vnt1.1 的鉴定和克隆，Rpi-vnt1.1 是一种以前未表征的文丘里茄晚疫病抗性基因。该基因通过经典的遗传和物理作图方法鉴定，编码与 S 的 Tm-22 高度相似的卷曲螺旋核苷酸结合富含亮氨酸的重复蛋白。 lycopersicum，赋予对番茄花叶病毒的抵抗力。携带Rpi-vnt1.1的转基因马铃薯和番茄植物被证明对致病疫霉具有抗性。 11P的。在infestansisolates测试中，只有来自厄瓜多尔的EC1分离株能够克服Rpi-vnt1.1并在接种的植物上引起疾病。在其他抗晚疫病材料中也发现了仅有几个核苷酸差异的Rpi-vnt1.1（Rpi-vnt1.2和Rpi-vnt1.3）等位基因。文丘里。来自S的晚疫病抗性基因Rpi-phu1。此处显示的 phurejais 与 Rpi-vnt1.1 相同，表明由于枯萎病抗性的选择压力，这种强抗性基因自共同祖先以来就一直保留下来，或者表明 S.phurejais 与 Rpi-vnt1.1 之间的遗传交换。文丘里管和S. phurejahas 在某个时候发生过。
Despite the efforts of breeders and the extensive use of fungicide control measures, late blight still remains a major threat to potato cultivation worldwide. The introduction of genetic resistance into cultivated potato is considered a valuable method to achieve durable resistance to late blight. Here, we report the identification and cloning ofRpi-vnt1.1, a previously uncharacterized late-blight resistance gene fromSolanum venturii. The gene was identified by a classical genetic and physical mapping approach and encodes a coiled-coil nucleotide-binding leucine-rich repeat protein with high similarity toTm-22fromS. lycopersicumwhich confers resistance againstTomato mosaic virus. Transgenic potato and tomato plants carryingRpi-vnt1.1were shown to be resistant toPhytophthora infestans. Of 11P. infestansisolates tested, only isolate EC1 from Ecuador was able to overcomeRpi-vnt1.1and cause disease on the inoculated plants. Alleles ofRpi-vnt1.1(Rpi-vnt1.2andRpi-vnt1.3) that differed by only a few nucleotides were found in other late-blight-resistant accessions ofS. venturii. The late blight resistance geneRpi-phu1fromS. phurejais shown here to be identical toRpi-vnt1.1, suggesting either that this strong resistance gene has been maintained since a common ancestor, due to selection pressure for blight resistance, or that genetic exchange betweenS. venturiiandS. phurejahas occurred at some time.