Differential cell-type dependent brain state modulations of sensory representations in the non-lemniscal mouse inferior colliculus
Differential cell-type dependent brain state modulations of sensory representations in the non-lemniscal mouse inferior colliculus
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DOI：
10.1038/s42003-019-0602-4
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发表时间：
2019-09-30
影响因子：
5.9
通讯作者：
Song, Sen
中科院分区：
文献类型：
--
作者：
Chen, Chenggang;Song, Sen
新皮层的感觉反应受到大脑状态变化的强烈影响。然而，目前尚不清楚中脑的感觉反应是否以及如何受到影响。在这里，我们解决了这个问题，通过使用在体双光子钙成像监测小鼠下丘（IC）的自发和声音诱发的活动。我们开发了一种方法，使我们能够在清醒的行为小鼠的非丘系IC（IC壳L1）的第一层图像。与清醒状态相比，异氟烷麻醉期间兴奋性神经元的光谱调谐选择性降低。行为动物的钙离子成像显示，抑制性神经元的活动与运动高度相关。与静止期相比，运动期兴奋性神经元的光谱调谐选择性增加。总之，我们的研究表明，在IC壳L1的神经元活动是大脑状态依赖性的，而大脑状态调制的兴奋性和抑制性神经元的差异。
Sensory responses of the neocortex are strongly influenced by brain state changes. However, it remains unclear whether and how the sensory responses of the midbrain are affected. Here we addressed this issue by using in vivo two-photon calcium imaging to monitor the spontaneous and sound-evoked activities in the mouse inferior colliculus (IC). We developed a method enabling us to image the first layer of non-lemniscal IC (IC shell L1) in awake behaving mice. Compared with the awake state, spectral tuning selectivity of excitatory neurons was decreased during isoflurane anesthesia. Calcium imaging in behaving animals revealed that activities of inhibitory neurons were highly correlated with locomotion. Compared with stationary periods, spectral tuning selectivity of excitatory neurons was increased during locomotion. Taken together, our studies reveal that neuronal activities in the IC shell L1 are brain state dependent, whereas the brain state modulates the excitatory and inhibitory neurons differentially.