Discovery of sandwich type COVID-19 nucleocapsid protein DNA aptamers
Discovery of sandwich type COVID-19 nucleocapsid protein DNA aptamers
复制标题
DOI：
10.1039/d0cc03993d
复制
发表时间：
2020-09-11
影响因子：
4.9
通讯作者：
Luo, Zhaofeng
中科院分区：
文献类型：
--
作者：
Zhang, Liyun;Fang, Xiaona;Luo, Zhaofeng
在这里，我们首次报道了靶向COVID-19核衣壳蛋白（Np）的DNA适体。Np是最丰富的结构蛋白之一，它可作为准确和灵敏检测COVID-19的诊断标志物。经过五轮筛选，我们获得了四个亲和力低于5 nM的DNA序列。最好的一个显示出对Np的极好的结合性能，aK（d）值为0.49 nM。有趣的是，我们发现这四对适体可以连续地与Np结合，这表明了一种双链型相互作用。利用这些夹心适体在ELISA和胶体金免疫层析条，我们能够检测在几十pM水平的Np。结果表明，适体是病毒检测、诊断和抗病毒治疗的有力分子工具。
Here, we report for the first time DNA aptamers targeted toward the COVID-19 nucleocapsid protein (Np). Np is one of the most abundant structural proteins and it serves as a diagnostic marker for the accurate and sensitive detection of COVID-19. After five rounds of selection, we obtained four DNA sequences with an affinity below 5 nM. The best one displayed a superb binding performance toward Np with aK(d)value of 0.49 nM. Interestingly, we found that the four pairs of aptamers could bind to Np successively, suggesting a sandwich-type interaction. Using these sandwiched aptamers in ELISA and colloidal gold immunochromatographic strips, we were able to detect Np at the tens of pM level. The results demonstrate that aptamers are powerful molecular tools for virus detection, diagnosis, and antiviral therapy.