Availability of porphobilinogen controls appearance of porphobilinogen deaminase activity in Escherichia coli K-12
Availability of porphobilinogen controls appearance of porphobilinogen deaminase activity in Escherichia coli K-12
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胆色素原的可用性控制大肠杆菌 K-12 中胆色素原脱氨酶活性的出现
DOI：
10.1128/jb.170.10.4969-4971.1988
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发表时间：
1988
影响因子：
3.2
通讯作者：
S. Cosloy
中科院分区：
文献类型：
--
作者：
H. Umanoff;C. S. Russell;S. Cosloy
一种血红素可透过的hemB突变体没有5-氨基乙酰丙酸酯酶（ALA D）和极低的胆色素原脱氨酶（PBG D）活性。当hemB的结构基因被引入到该菌株的单拷贝质粒上时，观察到两种活性。当突变体在PBG上生长时，观察到正常的PBG D活性。此外，除非在ALA或PBG上生长，否则hemA突变体几乎没有或没有PBG D活性。无论是氯化血红素，也没有PBG影响PBG D蛋白的水平产生的体外转录和翻译的质粒携带hemC基因作为插入。我们的结论是，在大肠杆菌中，PBG可用性控制PBG D的活性在转录后水平。
A hemin-permeable hemB mutant had no 5-aminolevulinate dehydratase (ALA D) and extremely low porphobilinogen deaminase (PBG D) activity. When the structural gene for hemB was introduced into this strain on a single-copy plasmid, both activities were observed. When the mutant was grown on PBG, normal PBG D activity was observed. Moreover, a hemA mutant had little or no PBG D activity unless it was grown on ALA or PBG. Neither hemin nor PBG affected the level of PBG D protein produced from in vitro transcription and translation of a plasmid harboring the hemC gene as an insert. We conclude that, in Escherichia coli, PBG availability controls the activity of PBG D at some posttranscriptional level.