Probing organization and communication at layered interfaces
Probing organization and communication at layered interfaces
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DOI：
10.1016/j.bioelechem.2006.06.002
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发表时间：
2007-05-01
影响因子：
5
通讯作者：
Blanchard, G. J.
中科院分区：
文献类型：
--
作者：
Dominska, Monika;Mazur, Maciej;Blanchard, G. J.
我们用电化学和光谱方法研究了各种界面结构所固有的局域结构。我们的重点一直放在仿生界面的设计和构建上，在仿生界面上可以结合脂质双分子层或杂化双分子膜。这项工作的最终目标是在换能器表面创建一个能够支持活性形式的酶的界面。在这一点上，我们已经研究了在将双层结构结合到换能器表面的单层中可实现的组织程度。我们的电化学数据表明，衬底表面在决定附着层组织方面起着重要作用。我们还利用时间分辨和稳态荧光光谱研究了脂质双层的流动性和结构异质性。我们的数据指出了脂质双层成分的高度相互作用的性质，其中对一个区域的扰动会对双层的其他区域产生重大影响。这些信息与模型和生物双层中脂筏结构的存在和性质直接相关。(C)2006爱思唯尔B.V.保留所有权利。
We have investigated the local organization intrinsic to a variety of interfacial structures, by both electrochemical and spectroscopic means. Our focus has been on the design and construction of biomimetic interfaces, where a lipid bilayer or a hybrid bilayer membrane can be bound to an interface. The goal of this work is ultimately to create an interface on a transducer surface that can support an enzyme in its active form. To this point, we have examined the extent of organization that is achievable in monolayers that will be used to bind bilayer structures to a transducer surface. Our electrochemical data point to the important role of the substrate surface in determining adlayer organization. We have also investigated the fluidity and structural heterogeneity of lipid bilayers using time-resolved and steady state fluorescence spectroscopy. Our data point to the highly interactive nature of lipid bilayer constituents, where perturbations introduced to one region have significant consequences on other regions of the bilayer. Such information is directly relevant to the existence and properties of lipid raft structures in both model and biological bilayers. (c) 2006 Elsevier B.V. All rights reserved.