MOUSE GATA-4 - A RETINOIC ACID-INDUCIBLE GATA-BINDING TRANSCRIPTION FACTOR EXPRESSED IN ENDODERMALLY DERIVED TISSUES AND HEART
MOUSE GATA-4 - A RETINOIC ACID-INDUCIBLE GATA-BINDING TRANSCRIPTION FACTOR EXPRESSED IN ENDODERMALLY DERIVED TISSUES AND HEART
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DOI：
10.1128/mcb.13.4.2235
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发表时间：
1993-04-01
影响因子：
5.3
通讯作者：
WILSON, DB
中科院分区：
文献类型：
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作者：
ARCECI, RJ;KING, AAJ;WILSON, DB
我们报道了小鼠GATA - 4的cDNA克隆及特性，它是结合核心GATA基序的锌指转录因子家族的一个新成员。通过用对应于指结构域高度保守区域的寡核苷酸探针筛选6.5天的小鼠胚胎文库，鉴定出了GATA - 4 cDNA。与该家族的其他蛋白质一样，GATA - 4大小约为50 kDa，包含两个形式为C - X - N - C - (X17) - C - N - X - C的锌指结构域。在异源细胞中的共转染实验表明，GATA - 4可激活含有GATA启动子元件的报告基因构建体。Northern（RNA）分析和原位杂交显示，GATA - 4 mRNA在心脏、肠上皮、原始内胚层和性腺中表达。维甲酸诱导小鼠F9细胞分化为内脏或壁内胚层伴随着GATA - 4 mRNA和蛋白质表达的增加。胚胎干细胞体外分化为拟胚体也与GATA - 4表达增加有关。我们得出结论，GATA - 4是一种组织特异性、维甲酸诱导且受发育调控的转录因子。基于其组织分布，我们推测GATA - 4在心脏、肠上皮、原始内胚层和性腺的基因表达中起作用。
We report the cDNA cloning and characterization of mouse GATA-4, a new member of the family of zinc finger transcription factors that bind a core GATA motif. GATA-4 cDNA was identified by screening a 6.5-day mouse embryo library with oligonucleotide probes corresponding to a highly conserved region of the finger domains. Like other proteins of the family, GATA-4 is approximately 50 kDa in size and contains two zinc finger domains of the form C-X-N-C-(X17)-C-N-X-C. Cotransfection assays in heterologous cells demonstrate that GATA-4 trans activates reporter constructs containing GATA promoter elements. Northern (RNA) analysis and in situ hybridization show that GATA-4 mRNA is expressed in the heart, intestinal epithelium, primitive endoderm, and gonads. Retinoic acid-induced differentiation of mouse F9 cells into visceral or parietal endoderm is accompanied by increased expression of GATA-4 mRNA and protein. In vitro differentiation of embryonic stem cells into embryoid bodies is also associated with increased GATA-4 expression. We conclude that GATA-4 is a tissue-specific, retinoic acid-inducible, and developmentally regulated transcription factor. On the basis of its tissue distribution, we speculate that GATA-4 plays a role in gene expression in the heart, intestinal epithelium, primitive endoderm, and gonads.