Intraocular tetrodotoxin in goldfish hinders optic nerve regeneration
Intraocular tetrodotoxin in goldfish hinders optic nerve regeneration
复制标题
金鱼眼内河豚毒素阻碍视神经再生
DOI：
10.1016/0006-8993(83)90957-5
复制
发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
B. Grafstein
中科院分区：
文献类型：
--
作者：
D. Edwards;B. Grafstein
重复眼内注射河豚毒素（TTX），以产生一个维持的神经活动的阻断在视神经被压碎后再生的海狗视轴突。在TTX处理的鱼的视觉功能的恢复延迟，表现为延迟返回的惊吓反应突然照明，背光反射和食物颗粒的本地化。在视神经挤压时单次注射TTX延迟惊吓反应的恢复，但不延迟食物定位的恢复。在TTX处理的动物中没有神经节细胞的损失，但是通过光学显微镜可检测到的再生轴突的数量减少。此外，在一些再生轴突中放射性标记蛋白的轴突运输在损伤后21-28天显示出缺陷，即，在顶盖的神经支配过程中在同一时期，标记的氨基酸在视网膜神经节细胞中的蛋白质掺入被抑制，但运输和掺入已恢复正常的36天后病变。这些结果，连同TTX 58，59的影响的伴随电生理学分析的结果，表明TTX治疗干扰两个独立的事件在再生，一个发生后不久的神经损伤和其他在神经支配的顶盖。在这些事件中的至少第一个事件期间，TTX治疗的效果可以是减少再生轴突分支的数量或大小。我们建议，TTX效应可能是介导的轴突运输的某些材料，包括神经节苷脂，核苷，或蛋白质，特别是参与再生的减少。
Repeated intraocular injection of tetrodotoxin (TTX) was use to produced a maintained block of neural activity in golfish optic axons which were regenerating following a crush of the optic nerve. The recovery of visual function was delayed in the TTX-treated fish, as demonstrated by delays in the return of the startle reaction to sudden illumination, the dorsal light reflex and food pellet localization. A single injection of TTX at the time of optic nerve crush delayed recovery of the startle reaction but not of food localization. There was no loss of ganglion cells in the TTX-treated animals, but the number of regenerated axons detectable by light microscopy was reduced. Also, axonal transport of radioactively labeled protein in some of the regenerating axons showed a deficit at 21–28 days after the lesion, i.e., during innervation of the tectum. Incorporation of labeled amino acid into protein in the retinal ganglion cells was depressed during the same period, but both the transport and incorporation had returned to normal by 36 days after the lesion. These results, together with the results of the accompanying electrophysiological analysis of the effects of TTX58,59, suggest that TTX treatment interferes with two separate events in regeneration, one occurring soon after the nerve lesion and the other during innervation of the tectum. During at least the first of these events the effect of TTX treatment may be to reduce the number or size of the regenerating axon branches. We propose that the TTX effect may be mediated by a reduction in the axonal transport of certain materials, including gangliosides, nucleosides, or proteins specifically involved in regeneration.
影响因子：
56.9
作者：
PURVES, D;LICHTMAN, JW
通讯作者：
LICHTMAN, JW