Automatic dendritic spine analysis in two-photon laser scanning microscopy images
Automatic dendritic spine analysis in two-photon laser scanning microscopy images
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DOI：
10.1002/cyto.a.20431
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发表时间：
2007-10-01
期刊：
影响因子：
3.7
通讯作者：
Wong, Stephen T. C.
中科院分区：
文献类型：
--
作者：
Bai, Wenjia;Zhou, Xiaobo;Wong, Stephen T. C.
树突棘表达在中枢神经系统中起重要作用。现代荧光显微镜和绿色荧光蛋白技术的发展为脊椎动物的研究提供了便利。为了定量分析荧光显微图像中的树突棘，提出了一种自动树突棘分析方法。由于轴向分辨率的限制，我们的方法被设计用于处理沿着z轴的投影图像并分析侧脊柱。该方法可以自动提取枝晶中心线，并根据基于宽度的准则沿枝晶沿着分割脊。该标准利用棘的共同形态特征。它可以检测到以前的方法错过的一些形状的脊椎。此外，所提出的方法是自动的，一旦几个参数的设置。生物学家感兴趣的脊柱数量、长度和密度都可以手动和自动分析。两种方法的结果吻合良好。所提出的方法提供了自动和准确的树突棘分析。它可以作为一个有用的工具，脊柱图像分析，以避免繁琐的手工劳动。(C)2007年国际分析细胞学学会。
Dendritic spine expression plays an important role in the central nervous system. Modern fluorescence microscopy and green fluorescent protein technology have facilitated the research on spines. To quantitatively analyze the spines in fluorescence microscopy images, an automatic dendritic spine analysis method is proposed. Because of the limit of axial resolution, our method is designed to process the projection image along the z-axis and analyze the lateral spines. The method can automatically extract the dendrite centerlines and segment the spines along the dendrites according to width-based criteria. The criteria utilize a common morphological feature of the spines. It can detect some shapes of spines missed by previous methods. In addition, the proposed method is automatic once a few parameters are set. Spine numbers, lengths, and densities, which biologists are interested in, are analyzed both manually and automatically. The results of the two methods match well. The proposed method provides automatic and accurate dendritic spine analysis. It can serve as a useful tool for spine image analysis to avoid tedious manual labor. (C) 2007 International Society for Analytical Cytology.