Characterization of complementary deoxyribonucleic acid and genomic deoxyribonucleic acid for the beta chain of human fibrinogen.
Characterization of complementary deoxyribonucleic acid and genomic deoxyribonucleic acid for the beta chain of human fibrinogen.
复制标题
人纤维蛋白原 β 链的互补脱氧核糖核酸和基因组脱氧核糖核酸的表征。
DOI：
10.1021/bi00282a032
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发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
Davie,EW
中科院分区：
文献类型：
--
作者：
Chung,DW;Que,BG;Rixon,MW;MaceJr,M;Davie,EW
Dominic W. Chung, Benito G. Que, Mark W. Rixon, Myles Mace, Jr., and Earl W. Davie* abstract: A total of 148 cDNAs coding for the 8 chain of human fibrinogen have been identified from a human liver cDNA library employing a bovine cDNA as a probe. The largest cDNA insert contained 1932 base pairs cloned into the Pstl site of plasmid pBR322. This cDNA insert contained 66 base pairs coding for a portion or all of a signal sequence, 1383 base pairs coding for 461 amino acids in the mature protein, a stop codon of TAG, a noncoding region of 431 base pairs, and a poly (A) tail of 19 base pairs. Most of the cDNA inserts coding for the 8 chain were found to have a noncoding region of 98 or 167base pairs rather than 431 base pairs at the 3'-end. The bovine cDNA for the 8 chain was also em-ployed as a probe for screening a X phage library containing
