The E3 ligase XBAT35 mediates thermoresponsive hypocotyl growth by targeting ELF3 for degradation in Arabidopsis
The E3 ligase XBAT35 mediates thermoresponsive hypocotyl growth by targeting ELF3 for degradation in Arabidopsis
复制标题
E3 连接酶 XBAT35 通过靶向 ELF3 降解拟南芥来介导热响应性下胚轴生长
DOI：
10.1111/jipb.13107
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发表时间：
2021-06-08
影响因子：
11.4
通讯作者：
Liu, Jian-Xiang
中科院分区：
文献类型：
--
作者：
Zhang, Lin-Lin;Li, Wei;Liu, Jian-Xiang
植物能够协调它们的生长和发育与环境温度。早花3(ELF3)是植物生物钟的重要组成部分，也参与环境温度的感知，以及抑制温度反应调节因子光敏色素相互作用因子4(PIF4)的表达和蛋白活性。ELF3的活性在温暖的温度下会受到衰减；然而，ELF3的蛋白质水平在温暖的温度下是如何调节的仍然知之甚少。在这里，我们报告了拟南芥中的E3连接酶XB3同源基因5，XBAT35，介导ELF3的降解。XBAT35与ELF3相互作用，泛化ELF3。XBAT35的功能缺失突变增加了ELF3的蛋白水平，并在温暖的温度条件下提供了一种短下胚轴表型。因此，我们的发现表明，XBAT35介导ELF3的降解，从而解除ELF3对PIF4的抑制，从而促进植物对温度敏感的下胚轴生长。
Plants are capable of coordination of their growth and development with ambient temperatures. EARLY FLOWERING3 (ELF3), an essential component of the plant circadian clock, is also involved in ambient temperature sensing, as well as in inhibiting the expression and protein activity of the thermoresponsive regulator phytochrome interacting factor 4 (PIF4). The ELF3 activity is subjected to attenuation in response to warm temperature; however, how the protein level of ELF3 is regulated at warm temperature remains less understood. Here, we report that the E3 ligase XB3 ORTHOLOG 5 IN ARABIDOPSIS THALIANA, XBAT35, mediates ELF3 degradation. XBAT35 interacts with ELF3 and ubiquitinates ELF3. Loss-of-function mutation of XBAT35 increases the protein level of ELF3 and confers a short-hypocotyl phenotype under warm temperature conditions. Thus, our findings establish that XBAT35 mediates ELF3 degradation to lift the inhibition of ELF3 on PIF4 for promoting thermoresponsive hypocotyl growth in plants.