SIMULTANEOUS LABELING OF VAGAL INNERVATION OF THE GUT AND AFFERENT-PROJECTIONS FROM THE VISCERAL FOREBRAIN WITH DIL INJECTED INTO THE DORSAL VAGAL COMPLEX IN THE RAT
SIMULTANEOUS LABELING OF VAGAL INNERVATION OF THE GUT AND AFFERENT-PROJECTIONS FROM THE VISCERAL FOREBRAIN WITH DIL INJECTED INTO THE DORSAL VAGAL COMPLEX IN THE RAT
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DOI：
10.1002/cne.903010107
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发表时间：
1990-11-01
影响因子：
2.5
通讯作者：
POWLEY, TL
中科院分区：
文献类型：
--
作者：
BERTHOUD, HR;JEDRZEJEWSKA, A;POWLEY, TL
用荧光碳花青染料 Dil 注射到迷走神经背侧运动核 (dmnX) 中，识别大鼠胃肠壁不同层的迷走神经支配。使用多次双侧注射来标记所有 dmnX 节前运动神经元，因此，终止于邻近孤束核 (nts) 的大多数迷走神经初级传入神经也被逆行和跨神经节标记。通过使用 Fluorogold 完全且特异性地标记肠神经系统，Dil 标记的迷走神经轮廓可以在其与肌间神经节和粘膜下神经节神经元的解剖关系中可视化和量化。在肌间神经丛中，迷走神经纤维和末端遍布整个胃肠道，一直到降结肠尾部，但迷走神经支配密度的近远侧梯度普遍下降。胃肌间丛的所有部分（胃底、胃体、胃窦）以及近端十二指肠都受到极其密集的神经支配，几乎每个神经节和结缔组织中都有迷走神经纤维和末端。此外，在尾部，受神经支配的肌间神经节的百分比和神经节内标记的平均密度都迅速下降，但盲肠和近端结肠除外，其中高达 65% 的神经节受到神经支配。在胃和十二指肠粘膜下层中很少发现迷走神经纤维和末端，并且在粘膜中没有发现迷走神经纤维和末端。使用正常的落射荧光和激光扫描共聚焦显微镜，可以识别各种尺寸和几何形状的高度静脉曲张或珠状末端结构。 Dil 注射浸渍了 dmnX 以及邻近的 nts，导致所有先前报道的前脑与背侧迷走神经复合体的连接发生逆行和顺行标记。我们得出的结论是，肌间神经丛是整个胃肠道迷走神经支配的主要目标，并且其神经支配比以前假设的更完整。相反，粘膜和粘膜下层的迷走神经传入（和传入）神经支配似乎明显稀疏或缺失。此外，使用更多 Dil 的局部注射为同时识别迷走神经节前的特定子集及其中枢神经输入提供了前景。
The vagal innervation of the different layers of the rat gastrointestinal wall was identified with the fluorescent carbocyanine dye Dil, injected into the dorsal motor nucleus of the vagus (dmnX). Multiple, bilateral injections were used to label all dmnX preganglionic motoneurons, and as a consequence, most of the vagal primary afferents that terminate in the adjacent nucleus of the solitary tract (nts) were also retrogradely and transganglionically labeled. With Fluorogold used to label the enteric nervous system completely and specifically, the Dil-labeled vagal profiles could be visualized and quantified in their anatomical relation to the neurons of the myenteric and submucous ganglia. In the myenteric plexus, vagal fibers and terminals were found throughout the gastrointestinal tract as far caudal as the descending colon, but there was a general decreasing proximodistal gradient in the density of vagal innervation. All parts of the gastric myenteric plexus (fundus, corpus, antrum), as well as the proximal duodenum, were extremely densely innervated, with vagal fibers and terminals in virtually every ganglion and connective. Further caudally, both the percentage of innervated myenteric ganglia and the average density of label within the ganglia rapidly decreased, with the exception of the cecum and proximal colon, where up to 65% of the ganglion were innervated. In the gastric and duodenal submucosa very few and in the mucosa no vagal fibers and terminals were found. With both normal epifluorescence and laser scanning confocal microscopy, highly varicose or beaded terminal structures of various size and geometry could be identified. The Dil injections, which impregnated the dmnX as well as the adjacent nts, resulted in retrograde and anterograde labeling of all the previously reported forebrain connections with the dorsal vagal complex. We conclude that the myenteric plexus is the primary target of vagal innervation throughout the gastrointestinal tract, and that its innervation is more complete than previously assumed. In contrast, vagal afferent (and afferent) innervation of mucosa and submucosa seems conspicuously sparse or absent. Furthermore, the use of more focal injections of Dil offers the prospect to simultaneously identify specific subsets of vagal preganglionics and their central nervous inputs.