High resolution ordering of YAC contigs using extended chromatin and chromosomes.
High resolution ordering of YAC contigs using extended chromatin and chromosomes.
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DOI：
10.1093/hmg/3.4.629
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发表时间：
1994-04
影响因子：
3.5
通讯作者：
Thomas Haaf;David C. Ward
中科院分区：
文献类型：
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作者：
Thomas Haaf;David C. Ward
荧光原位杂交（FISH）对人类基因组物理图谱的普遍有用性由于DNA分辨率的提高而大大增强。已经描述了几种以线性方式去致密化或拉伸间期染色质的技术，允许更精细的远程FISH映射。高度延伸的线性染色质可以在至少几个百万碱基的物理距离上杂交，可能在染色体的整个长度上杂交。通过多色FISH，我们从5q34-35序列中确定了YACs的顺序和重叠。cosmid可以定位在更大的YAC克隆中。扩展染色质作图可以作为一种辅助排序技术应用于基因组研究。
The general usefulness of fluorescence in situ hybridization (FISH) for the physical mapping of the human genome is greatly enhanced by improved DNA resolution. Several techniques have been described for decondensing or stretching interphase chromatin in a linear fashion, allowing long-range FISH mapping in finer detail. Highly extended linear chromatin can be hybridized over physical distances of at least several megabases, possibly over the whole length of a chromosome. By multi-color FISH, we have determined the order and overlaps of YACs from a 5q34-35 contig. Cosmids can be localized within larger YAC clones. Extended chromatin mapping can be applied as an adjunct ordering technique in genome studies.