Immunological detection of muscarinic receptor subtype proteins (m1-m5) in rabbit peripheral tissues.
Immunological detection of muscarinic receptor subtype proteins (m1-m5) in rabbit peripheral tissues.
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DOI：
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发表时间：
1991-10
影响因子：
3.6
通讯作者：
F. Dörje;A. Levey;M. Brann
中科院分区：
文献类型：
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作者：
F. Dörje;A. Levey;M. Brann
使用亚型特异性抗血清，我们测量了兔外周组织中五种毒蕈碱受体亚型蛋白（m1-m5）的相对水平。免疫沉淀分析表明存在四种不同的受体蛋白 (m1-m4)，其组织分布存在显着差异。在任何检查的组织中均未检测到大量 m5 受体。在交感神经节和颌下腺中发现高水平的 m1 受体。 m1 受体是输精管中的主要亚型。 m2 型毒蕈碱受体以不同比例存在于所有检查的组织中，是交感神经节、回肠和子宫中的主要亚型，也是在兔心房中检测到的唯一亚型。 m3 受体蛋白在颌下腺中含量丰富，但在研究的其他组织中未检测到明显水平。 m4型受体代表了周围肺中的主要亚型，并且也存在于子宫和回肠组织中，尽管水平较低。
Employing subtype-specific antisera, we have measured the relative levels of five muscarinic receptor subtype proteins (m1-m5) in rabbit peripheral tissues. Immunoprecipitation assays demonstrated the presence of four distinct receptor proteins (m1-m4), which showed notable differences in their tissue distribution. Significant amounts of m5 receptors were not detected in any of the tissues examined. High levels of m1 receptors were found in sympathetic ganglia and submaxillary gland. The m1 receptor was the predominant subtype in vas deferens. Muscarinic receptors of the m2 type were present in varying proportions in all tissues examined, being the major subtype in sympathetic ganglia, ileum, and uterus and the only subtype detected in rabbit atrium. The m3 receptor protein was abundant in submaxillary gland but was not detected at appreciable levels in other tissues studied. Receptors of the m4 type represented a major subtype in peripheral lung and were also present, although at lower levels, in uterine and ileal tissues.