Activation and proliferation of follicular dendritic cell-like cells by activated T lymphocytes.
Activation and proliferation of follicular dendritic cell-like cells by activated T lymphocytes.
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DOI：
10.4049/jimmunol.153.7.2951
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发表时间：
1994-10
影响因子：
4.4
通讯作者：
Han Soo Kim;Xinhong Zhang;Y. Choi
中科院分区：
文献类型：
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作者：
Han Soo Kim;Xinhong Zhang;Y. Choi
建立人扁桃体滤泡树突状细胞样细胞系（HK细胞），研究FDC在淋巴滤泡生发中心的功能作用。虽然HK细胞在建立早期表现出在人扁桃体FDC上表达的CD21、CD23、dc -1、CD40、VCAM-1、ICAM-1和HJ2，但在培养3天后，它们就失去了dc -1、CD21和CD23。形态学和功能表征研究表明，HK细胞与成纤维细胞有很大的不同。HK细胞的生长需求不同于先前报道的fdc样细胞系。在功能上，这些细胞结合B细胞而不是T细胞，并支持B细胞增殖。HK细胞培养上清液对抗mu或抗cd40激活的B细胞增殖均有共刺激作用，且经12- o -十四烷醇- 13-乙酸刺激后活性显著增强。有趣的是，抗cd3 Ab激活了与HK细胞结合的T细胞，诱导了HK细胞的表型改变和生长。T-HK细胞间的相互作用不仅涉及众所周知的粘附配体/受体途径（vla4 /VCAM-1和LFA-1/ICAM-1），还涉及CD40-CD40配体，如可溶性CD40的抑制作用（CD40. fc）。T细胞和HK细胞之间的相互作用以及细胞因子的产生表明，活化的T细胞不仅可以直接刺激静止的B细胞，还可以通过刺激FDC的发育间接支持B细胞成熟。因此，HK细胞可能有助于识别参与GC形成的FDC的表面分子和细胞因子。
Follicular dendritic cell (FDC)-like cell lines (HK cells) from human tonsils were established to investigate the functional role of FDC in the germinal centers of lymphoid follicles. Although HK cells exhibited CD21, CD23, DRC-1, CD40, VCAM-1, ICAM-1, and HJ2 that were expressed on human tonsilar FDC at early stages of establishment, they lost DRC-1, CD21, and CD23 within 3 days of culture. Morphologic and functional characterization studies suggest that HK cells are quite distinct from fibroblasts. The growth requirement of HK cells is different from the previously reported FDC-like cell lines. Functionally, these cells bound B cells but not T cells, and supported B cell proliferation. The culture supernatants of HK cells had costimulatory activity on the proliferation of anti-mu- or anti-CD40-activated B cells, and the activity dramatically increased after 12-O-tetradecanoylphorbol 13-acetate stimulation. Interestingly, anti-CD3 Ab activated T cell bound to HK cells, inducing the phenotypic changes and the growth of HK cells. The T-HK cell interactions not only involve the well known adhesion ligand/receptor pathways (VLA-4/VCAM-1 and LFA-1/ICAM-1), but also involve CD40-CD40 ligand as shown by inhibitory effect of soluble CD40 (CD40.Fc). The cellular interactions between T and HK cells and cytokine production suggest that activated T cells not only stimulate resting B cells directly, but also support B cell maturation indirectly by stimulating the development of FDC. Hence HK cells may be useful in identifying the surface molecules and cytokines of FDC involved in the GC formation.