Identification of quantitative trait loci for leaf-related traits in an IBM Syn10 DH maize population across three environments
Identification of quantitative trait loci for leaf-related traits in an IBM Syn10 DH maize population across three environments
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DOI：
10.1111/pbr.12566
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发表时间：
2018-04-01
期刊：
影响因子：
2
通讯作者：
Lubberstedt, Thomas
中科院分区：
文献类型：
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作者：
Ma, Langlang;Guan, Zhongrong;Lubberstedt, Thomas
与叶相关的性状(叶长、叶宽、叶面积和叶角)对玉米(L)的产量有重要影响，因为它们对株型有影响。因此，有必要对叶片相关性状进行数量性状基因定位。本文提供了221个IBM Syn10dh群体的加倍单倍体系(DHL)用于QTL定位。利用高密度遗传连锁图谱，在单一环境中共检测到54个与叶片相关的QTL。其中，在两个或三个环境中只检测到8个共同的QTL，4个性状的共同QTL解释了4.38%~19.99%的表型变异。以前的研究中检测到qll-2-1(bin 2.09)、qLW-2-2(bin 2.09)、qLW-6-3(bin 6.07)和qla-5-2(bin 2.09)，qll-1-1、qLAr-1-1、qLAr-2-1和qla-7-1可能是新的QTL。值得注意的是，qLW-6-3和qLA-5-2分别是解释表型变异的19.99%和10.96%的主效QTL。有趣的是，我们发现了三对控制不同性状的QTL(qLW-2-2和qLAR-2-1，qLW-8-1和qLL-8-2，qLL-3-3和qLAR-3-3)，它们位于同一染色体上或附近。此外，还发现了9对具有上位性效应的基因座。这些结果可能为QTL精细定位和了解叶片相关性状变异的遗传基础提供基础。
Leaf-related traits (leaf length, leaf width, leaf area and leaf angle) are very important for the yield of maize (Zea mays L) due to their influence on plant type. Therefore, it is necessary to identify quantitative trait loci (QTLs) for leaf-related traits. In this report, 221 doubled haploid lines (DHLs) of an IBM Syn10 DH population were provided for QTL mapping. In total, 54 QTLs were detected for leaf-related traits in single environments using a high-density genetic linkage map. Among them, only eight common QTLs were identified across two or three environments, and the common QTLs for the four traits explained 4.38%-19.99% of the phenotypic variation. qLL-2-1 (bin 2.09), qLW-2-2 (bin 2.09), qLW-6-3 (bin 6.07) and qLA-5-2 (bin 2.09) were detected in previous studies, and qLL-1-1, qLAr-1-1, qLAr-2-1 and qLA-7-1 may be new QTLs. Notably, qLW-6-3 and qLA-5-2 were found to be major QTLs explaining 19.99% and 10.96% of the phenotypic variation, respectively. Interestingly, we found three pairs of QTLs (qLW-2-2 and qLAr-2-1, qLW-8-1 and qLL-8-2, qLL-3-3 and qLAr-3-3) that control different traits and that were located on the same chromosome or in a nearby location. Moreover, nine pairs of loci with epistatic effects were identified for the four traits. These results may provide the foundation for QTL fine mapping and for an understanding of the genetic basis of variation in leaf-related traits.