Detection of seroconversion to West Nile virus, Usutu virus and Sindbis virus in UK sentinel chickens.
Detection of seroconversion to West Nile virus, Usutu virus and Sindbis virus in UK sentinel chickens.
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DOI：
10.1186/1743-422x-3-71
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发表时间：
2006-09-04
期刊：
影响因子：
4.8
通讯作者：
Gould EA
中科院分区：
文献类型：
--
作者：
Buckley A;Dawson A;Gould EA
我们之前报告了西尼罗河病毒(WNV)在英国鸟类中传播的证据，可能是由海外的候鸟传入的。我们现在展示了在英国农场饲养的哨兵鸡中的西尼罗河病毒特异性血清转换。孵化后的母体WNV中和抗体在三周内下降。在接下来的几个月里，健康的鸡产生了西尼罗河病毒中和抗体，这些抗体通过免疫印迹和使用西尼罗河病毒抗原的间接免疫荧光试验得到确认。西尼罗河病毒阳性鸡比例高于乌苏图病毒和辛德比斯病毒。分离传染性病毒或检测血清中病毒RNA的尝试失败了。
We previously reported evidence of West Nile virus (WNV) circulation in UK birds, probably introduced by migratory birds from overseas. We now demonstrate WNV-specific seroconversion in sentinel chickens raised on an English farm. Maternal neutralizing antibodies to WNV in hatchlings declined within three weeks. During the following months, healthy chickens developed WNV neutralizing antibodies that were confirmed by immunoblotting and indirect immunofluorescence tests using WNV antigens. The proportion of seropositive chickens was higher for WNV than for Usutu virus or Sindbis virus. Attempts to isolate infectious virus or to detect viral RNA in the sera, failed.