Reactive oxygen species from chloroplasts contribute to 3-acetyl-5-isopropyltetramic acid-induced leaf necrosis of Arabidopsis thaliana
Reactive oxygen species from chloroplasts contribute to 3-acetyl-5-isopropyltetramic acid-induced leaf necrosis of Arabidopsis thaliana
复制标题
叶绿体中的活性氧导致 3-乙酰基-5-异丙基四胺酸诱导拟南芥叶片坏死
DOI：
10.1016/j.plaphy.2011.11.004
复制
发表时间：
2012-03-01
影响因子：
6.5
通讯作者：
Qiang, Sheng
中科院分区：
文献类型：
--
作者：
Chen, Shiguo;Yin, Chunyan;Qiang, Sheng
3-乙酰基-5-异丙基四酸（3-AIPTA）是四酸的衍生物，是许多植物褐斑病的罪魁祸首，经常杀死单子叶和双子叶植物的幼苗。为了进一步阐明3-AIPTA的作用模式，在3-AIPTA诱导的细胞坏死过程中，我们进行了一系列实验来评估活性氧（ROS）在这一过程中的作用。3-AIPTA作用于拟南芥叶片，抑制了PSII光系统II (PSII的主要质体醌受体Q(A)外的电子传递和PSII受体侧末端受体的还原；随后，电荷重组和电子泄漏到O-2增加，导致叶绿体衍生的氧化破裂。主要抗氧化酶如超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）活性下降。过量的ROS分子直接攻击多种细胞成分，导致电解质泄漏、脂质过氧化和细胞膜破坏。最后，这导致细胞破坏和叶组织坏死。因此，3- aipta引发的拟南芥叶片坏死被发现是叶绿体起源的ROS爆发直接氧化损伤的结果，这是由于抑制正常的光合电子传递而引起的。(C) 2011 Elsevier Masson SAS。版权所有。
3-Acetyl-5-isopropyltetramic acid (3-AIPTA), a derivate of tetramic acid, is responsible for brown leaf-spot disease in many plants and often kills seedlings of both mono- and dicotyledonous plants. To further elucidate the mode of action of 3-AIPTA, during 3-AIPTA-induced cell necrosis, a series of experiments were performed to assess the role of reactive oxygen species (ROS) in this process. when Arabidopsis thaliana leaves were incubated with 3-AIPTA, photosystem II (PSII) electron transport beyond Q(A) (the primary plastoquinone acceptor of PSII) and the reduction of the end acceptors at the PSI acceptor side were inhibited; this was followed by increase in charge recombination and electron leakage to O-2, resulting in chloroplast-derived oxidative burst. Furthermore, the main antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) lost their activity. Excess ROS molecules directly attacked a variety of cellular components and subsequently caused electrolyte leakage, lipid peroxidation and cell membrane disruption. Finally, this led to cell destruction and leaf tissue necrosis. Thus, 3-AIPTA-triggered leaf necrosis of Arabidopsis was found to be a result of direct oxidative injury from the chloroplast-originated ROS burst initiated by the inhibition of normal photosynthetic electron transport. (C) 2011 Elsevier Masson SAS. All rights reserved.