CIRCADIAN CLOCK LOCUS FREQUENCY - PROTEIN ENCODED BY A SINGLE OPEN READING FRAME DEFINES PERIOD LENGTH AND TEMPERATURE COMPENSATION
CIRCADIAN CLOCK LOCUS FREQUENCY - PROTEIN ENCODED BY A SINGLE OPEN READING FRAME DEFINES PERIOD LENGTH AND TEMPERATURE COMPENSATION
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DOI：
10.1073/pnas.91.16.7683
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发表时间：
1994-08-02
影响因子：
11.1
通讯作者：
DUNLAP, JC
中科院分区：
文献类型：
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作者：
ARONSON, BD;JOHNSON, KA;DUNLAP, JC
频率（frq）轨迹编码一个关键组成部分，状态变量，在细胞振荡器产生昼夜节律。两个成绩单已被映射到这个区域，这里提供的数据与第三个成绩单的存在是一致的。从这个区域的cDNA克隆和时钟突变体的分析集中在一个转录编码蛋白质。FRQ是一个中心时钟组分：（i）所有半显性frq等位基因中的突变是单个氨基酸取代的结果，并映射到编码FRQ的开放阅读框（ORF）;（ii）该ORF的缺失或其中的移码突变导致菌株具有隐性时钟表型，其特征在于节律稳定性和补偿的丧失。因此，该单个ORF内的单个氨基酸取代或破坏足以驱动周期长度和温度补偿这两个昼夜节律系统的典型特征的重大改变。由989个氨基酸组成的FRQ蛋白在脉孢菌生物码头的组装中指定了frq的昼夜节律功能。
The frequency (frq) locus encodes a key component, a state variable, in a cellular oscillator generating circadian rhythmicity. Two transcripts have been mapped to this region, and data presented here are consistent with the existence of a third transcript. Analysis of cDNA clones and clock mutants from this region focuses attention on one transcript encoding a protein. FRQ, which is a central clock component: (i) mutations in all of the semidominant frq alleles are the result of single amino acid substitutions and map to the open reading frame (ORF) encoding FRQ; (ii) deletion of this ORF, or a frameshift mutation within it, results in a strain with a recessive clock phenotype characterized by the loss of rhythm stability and compensation. Single amino acid substitutions within, or disruption of, this single ORF are thus sufficient to drive major alterations in both period length and temperature compensation, two canonical characteristics of circadian systems. The 989-amino acid FRQ protein specifies the circadian function of frq in the assembly of the Neurospora biological dock.