Discrimination of normal and malignant mucosal tissues of the colon by Raman Spectroscopy
Discrimination of normal and malignant mucosal tissues of the colon by Raman Spectroscopy
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DOI：
10.1089/pho.2006.2066
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发表时间：
2007-08-01
影响因子：
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通讯作者：
Mathew, Stanley
中科院分区：
文献类型：
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作者：
Chowdary, M. V. P.;Kumar, K. Kalyan;Mathew, Stanley
目的：本研究的目的是评估鉴别参数马氏距离、光谱残差和限限试验的适用性，该组开发的区分正常和恶性结肠组织。背景资料：结肠癌的诊断使用纤维内镜定位和随后的活检组织病理检查，这高度依赖于研究者的技能和经验。存在遗漏重要病变的风险，尤其是原位癌。拉曼光谱对样品中的生化变化敏感，适用于多变量统计工具，可以快速客观地检测结肠癌。方法：采用常规近红外（NIR）拉曼光谱（激发波长为785 nm）记录了11例正常和11例恶性离体结肠组织的102个光谱。采用主成分分析（PCA）、马氏距离、光谱残差等判别参数和多参数极限检验方法对光谱数据进行分析。结果：与正常组织相比，平均恶性光谱表现出相对较强的波段，表明存在额外的生物分子，如蛋白质（较强的酰胺III和I），脂质（1100,1300 cm(-1)）和DNA （1340,1470 cm(-1)）。平均正态光谱表明存在无序结构（峰位于1247 cm（-1）处）。因子1的分数给出了良好的判别，并通过使用马氏距离和光谱残差作为判别参数进一步微调。极限检验方法提供了明确的客观区分。结论：本研究进一步支持拉曼光谱结合限度试验鉴别正常和恶性结肠组织的有效性。多参数极限检验方法是用户友好的，临床医生或受过最低限度训练的个人可以直接将未知光谱与可用标准集进行比较，从而立即，客观，明确地做出决定。
Objective: The objective of this study was to evaluate the applicability of the discrimination parameters Mahalanobis distance, spectral residuals, and limit tests, developed by this group to differentiate normal from malignant colon tissues. Background Data: Colon cancers are diagnosed using fiberoptic endoscopic localization and a subsequent histopathological examination of biopsied tissue, which is highly dependent on the skill and experience of the investigator. There exists a risk of missing significant lesions, especially with carcinoma in situ lesions. Raman spectroscopy, which is sensitive to biochemical variations in the samples and amenable to multivariate statistical tools, can lead to rapid and objective detection of colon cancer. Methods: A total of 102 spectra from 11 normal and 11 malignant ex vivo colon tissues were recorded by conventional near infrared ( NIR) Raman spectroscopy ( excitation wavelength of 785 nm). Spectral data were analyzed by principal components analysis ( PCA) and other discriminating parameters, namely Mahalanobis distance, spectral residuals, and a multiparametric limit test approach. Results: Mean malignant spectra exhibit relatively stronger bands, suggesting the presence of additional biomolecules such as protein ( stronger amide III and I), lipids ( 1100, 1300 cm(-1)), and DNA ( 1340, 1470 cm(-1)) versus those seen in normal tissue. Mean normal spectra indicate the presence of disordered structures ( hump at 1247 cm(-1)). Scores of factor 1 gave good discrimination, and this is further fine-tuned by employing Mahalanobis distance and spectral residuals as discriminating parameters. A limit test approach provided unambiguous objective discrimination. Conclusion: This study further supports the efficacy of Raman spectroscopy, in combination with a limit test, for discrimination of normal and malignant colon tissues. The multiparametric limit test approach is user-friendly, and a clinician or minimally trained individual could directly compare the unknown spectra against the available standard sets to make the decision instantly, objectively, and unambiguously.