miR-183 induces cell proliferation, migration, and invasion by regulating PDCD4 expression in the SW1990 pancreatic cancer cell line
miR-183 induces cell proliferation, migration, and invasion by regulating PDCD4 expression in the SW1990 pancreatic cancer cell line
复制标题
miR-183通过调节SW1990胰腺癌细胞系中PDCD4的表达诱导细胞增殖、迁移和侵袭
DOI：
10.1016/j.biopha.2015.01.016
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发表时间：
2015-03-01
影响因子：
7.5
通讯作者：
Zeng, Yue
中科院分区：
文献类型：
--
作者：
Lu, Ying-Ying;Zheng, Jun-Yuan;Zeng, Yue
本研究的目的是研究miR-183在SW 1990癌细胞系中的功能，以及调控这些过程的机制。已知miRNA在癌细胞发育中起重要作用。然而，miR-183在胰腺癌中的模式和生物学作用在很大程度上仍然未知。在这里，我们已经报道了通过miR-183干预，通过诱导凋亡和降低Bcl-2表达，在体外胰腺癌细胞生长减少。此外，观察到miR-183增强胰腺癌细胞的迁移和侵袭，而抑制miR-183引起相反的效果。显示miR-183抑制增加E-钙粘蛋白表达并降低N-钙粘蛋白表达。这些调节作用在癌上皮-间质转化（EMT）中起重要作用。从机制上讲，我们证明了miR-183的过表达降低了PDCD 4（程序性细胞死亡4）mRNA和蛋白的表达，反之亦然。这有助于将PDCD 4确定为胰腺癌的靶基因。总之，我们的分析表明miR-183是细胞迁移的重要贡献者。这也可以用作胰腺癌治疗的潜在治疗靶点。(C)2015年Elsevier Masson SAS。All rights reserved.
The aim of this study was to investigate the function of miR-183 in the SW1990 cancer cell line, and the mechanisms regulating these processes. miRNAs are known to play important roles in cancer cell development. However, the pattern and biological role of miR-183 in pancreatic cancer remain largely unknown. Here, we have reported the reduction in pancreatic cancer cell growth in vitro by miR-183 intervention, by inducing apoptosis and decreasing the Bcl-2 expression. Moreover, miR-183 was observed to enhance pancreatic cancer cell migration and invasion, whereas inhibition of miR-183 caused an opposite effect. miR-183 inhibition was shown to increase E-cadherin expression and decrease N-cadherin expression. These regulatory actions play an important role in the cancer epithelial-mesenchymal transition (EMT). Mechanistically, we demonstrated that the overexpression of miR-183 decreased the expression of PDCD4 (programmed cell death 4) mRNA and protein, and vice versa. This helped to identify PDCD4 as the target genes in pancreatic cancer. In conclusion, our analyses indicated miR-183 to be an important contributor to cell migration. This could also be used as a potential therapeutic target for pancreatic cancer treatment. (C) 2015 Elsevier Masson SAS. All rights reserved.