How To Detect NDM-1 Producers
How To Detect NDM-1 Producers
复制标题
DOI：
10.1128/jcm.01773-10
复制
发表时间：
2011-02-01
影响因子：
9.4
通讯作者：
Walsh, Timothy R.
中科院分区：
文献类型：
--
作者：
Nordmann, Patrice;Poirel, Laurent;Walsh, Timothy R.
表达碳青霉烯酶 NDM-1 的肠杆菌分离株正在世界范围内出现。本研究纳入了来自世界各地的 27 个 NDM-1 阳性分离株，以鉴定这些菌株不仅是病原体，而且是正常菌群的定殖者，用于感染控制筛查。尽管对碳青霉烯类药物的敏感性各不相同，但联合测试（IMP/IMP + EDTA）、Etest MBL 和 Vitek2 (bioMerieux) 的自动敏感性测试确定了那些 NDM-1 生产者，并通过使用特定引物的 PCR 进行了验证。 NDM-1 生产者携带者的筛选可基于常规用于筛选广谱 β-内酰胺酶生产者的 ChromID ESBL 培养基等培养基，该培养基可提供出色的检测水平和低检测限（范围为 8 x 10(0) 至 5 x 10(2) CFU/ml）。科玛嘉 KPC 培养基具有较高的检测限（1 x 10(1) 至 5 x 10(5) CFU/ml），可用于 NDM-1 生产者感染暴发的跟踪。在这些筛选培养基上生长的菌落可以用本文所述的分子方法验证为NDM-1生产者。
Enterobacterial isolates expressing the carbapenemase NDM-1 are emerging worldwide. Twenty-seven NDM-1-positive isolates of worldwide origin were included in this study to identify these strains as not only pathogens but also colonizers of normal flora for infection control screening. Although susceptibility to carbapenems varied, a combined test (IMP/IMP + EDTA), the Etest MBL, and automated susceptibility testing by Vitek2 (bioMerieux) identified those NDM-1 producers as verified by PCR using specific primers. Screening for carriers of NDM-1 producers may be based on media such as the ChromID ESBL culture medium routinely used to screen for extended-spectrum beta-lactamase producers, which gives excellent detection levels with low limits of detection ranging from 8 x 10(0) to 5 x 10(2) CFU/ml. The CHROMagar KPC culture medium had higher limits of detection (1 x 10(1) to 5 x 10(5) CFU/ml) and may be proposed for the follow-up of outbreaks of infections with NDM-1 producers. Colonies growing on these screening media can be verified as NDM-1 producers with molecular methods as described herein.