Development and optimization of a lab-on-a-chip device for the measurement of trace nitrogen dioxide gas in the atmosphere.
Development and optimization of a lab-on-a-chip device for the measurement of trace nitrogen dioxide gas in the atmosphere.
复制标题
DOI：
10.1039/b513999f
复制
发表时间：
2006-03
期刊：
影响因子：
--
通讯作者：
Y. Takabayashi;M. Uemoto;Kenjiro Aoki;T. Odake;T. Korenaga
中科院分区：
文献类型：
--
作者：
Y. Takabayashi;M. Uemoto;Kenjiro Aoki;T. Odake;T. Korenaga
我们建议使用实验室芯片技术来测量气态化学污染物，并描述了用于检测空气中二氧化氮（NO2）的微芯片的开发。一种由石英玻璃制成的微芯片已被开发用于处理以下三种功能：气体吸收、化学反应和荧光检测。在微芯片中构建的通道用多孔玻璃板覆盖，允许二氧化氮渗透到在下面的微通道内流动的三乙醇胺（TEA）中。然后将二氧化氮与TEA混合，并在微芯片的化学反应室中与合适的荧光试剂反应。然后使反应后的溶液流入荧光检测区域，通过紫外发光二极管（UV-LED）激发，使用光电倍增管（PMT）检测荧光。对反应时间、试剂浓度、pH值、流速等测量条件进行了优化，用于空气中二氧化氮的分析。对标准化测试溶液的初步研究揭示了亚硝酸根离子（NO2-）的定量测量，其对应于10 - 80 ppbv范围内的大气二氧化氮。
We propose the use of lab-on-a-chip technology for measuring gaseous chemical pollutants, and describe the development of a microchip for the detection of nitrogen dioxide (NO2) in air. A microchip fabricated from quartz glass has been developed for handling the following three functions, gas absorption, chemical reaction and fluorescence detection. Channels constructed in the microchip were covered with porous glass plates, allowing nitrogen dioxide to penetrate into the triethanolamine (TEA) flowing within the microchannel beneath. The nitrogen dioxide was then mixed with TEA and reacted with a suitable fluorescence reagent in the chemical reaction chamber in the microchip. The reacted solution was then allowed to flow into the fluorescence detection area to be excited by an ultraviolet light-emitting diode (UV-LED), and the fluorescence was detected using a photomultiplier tube (PMT). The reaction time, reagent concentration, pH, flow rate and other measurement conditions were optimised for analysis of nitrogen dioxide in air. Preliminary studies with standardized test solutions revealed quantitative measurements of nitrite ion (NO2-), which corresponded to atmospheric nitrogen dioxide in the range of 10-80 ppbv.