Evidence that the receptor for soluble CD14:LPS complexes may not be the putative signal-transducing molecule associated with membrane-bound CD14.
Evidence that the receptor for soluble CD14:LPS complexes may not be the putative signal-transducing molecule associated with membrane-bound CD14.
复制标题
有证据表明，可溶性 CD14:LPS 复合物的受体可能不是与膜结合 CD14 相关的推定信号转导分子。
DOI：
10.1046/j.1365-3083.1997.d01-124.x
复制
发表时间：
1997
影响因子：
3.7
通讯作者：
Goyert,SM
中科院分区：
文献类型：
--
作者：
Haziot,A;Katz,I;Rong,GW;Lin,XY;Silver,J;Goyert,SM
膜结合CD 14作为单核细胞/巨噬细胞和中性粒细胞上脂多糖（LPS）的受体。研究表明，通过LPS与膜结合的CD 14结合激活单核细胞/巨噬细胞可能需要信号转导分子与膜结合的CD 14结合。然而，观察到非CD 14表达细胞（如内皮细胞）可以被LPS和可溶性形式的CD 14（sCD 14）的复合物激活，这表明该复合物的受体可能与膜结合CD 14相关的信号转导分子相同。所描述的研究表明，两种CD 14特异性MoAb能够阻断LPS诱导的内皮细胞活化，但不影响单核细胞对LPS的反应。这表明sCD 14：LPS复合物与内皮细胞的相互作用不同于膜结合CD 14与其推定的信号转导分子的相互作用。
Membrane‐bound CD14 acts as a receptor for lipopolysaccharide (LPS) on monocytes/macrophages and neutrophils. Studies have suggested that the activation of monocytes/macrophages by the binding of LPS to membrane‐bound CD14 may require the association of a signal‐transducing molecule with membrane‐bound CD14. The observation that non‐CD14 expressing cells, such as endothelial cells, can nevertheless be activated by a complex of LPS and a soluble form of CD14 (sCD14) suggests that the receptor for this complex may be identical to the signal transducing molecule associated with membrane‐bound CD14. The studies described show that two CD14‐specific MoAb are able to block the LPS‐induced activation of endothelial cells but do not affect the response of monocytes to LPS. This suggests that the interaction of the sCD14:LPS complex with endothelial cells is distinct from the interaction of membrane‐bound CD14 with its putative signal‐transducing molecule.