Characterization of peroxisome-deficient mutants of Hansenula polymorpha.
Characterization of peroxisome-deficient mutants of Hansenula polymorpha.
复制标题
多形汉逊酵母过氧化物酶体缺陷突变体的表征。
DOI：
10.1007/bf00309784
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发表时间：
1995
期刊：
影响因子：
2.5
通讯作者：
Cregg,JM
中科院分区：
文献类型：
--
作者：
Tan,X;Titorenko,VI;vanderKlei,IJ;Sulter,GJ;Haima,P;Waterham,HR;Eyers,M;Harder,W;Veenhuis,M;Cregg,JM
在甲基营养酵母汉逊酵母中，大约 25% 的甲醇利用缺陷 (Mut-) 突变体的过氧化物酶体生物发生所需的基因 (PERgenes) 受到影响。之前，我们报道了一组称为 Pim- 的置换突变体，其特征是存在一些小的过氧化物酶体，大部分过氧化物酶体酶位于细胞质中。在这里，我们描述了第二组主要的排列突变体，观察到它们缺乏任何过氧化物酶体样结构（Per-）。在每个全突变体中，过氧化物酶体甲醇途径酶醇氧化酶、过氧化氢酶和二羟丙酮合酶均存在且有活性，但位于胞质溶胶中。 Pim 和 Per-mutant 集合总共涉及 14 个不同 PER 基因的突变。其中两个基因 PER5 和 PER7 由显性失活和隐性等位基因代表。由显性菌株和野生型H杂交产生的二倍体。多态性突变并含有形态异常的过氧化物酶体。这是影响过氧化物酶体生物发生的显性失活突变的第一份报告。
In the methylotrophic yeastHansenula polymorpha, approximately 25% of all methanol-utilization-defective (Mut-) mutants are affected in genes required for peroxisome biogenesis (PERgenes). Previously, we reported that one group ofpermutants, termed Pim-, are characterized by the presence of a few small peroxisomes with the bulk of peroxisomal enzymes located in the cytosol. Here, we describe a second major group ofpermutants that were observed to be devoid of any peroxisome-like structure (Per-). In each Per-mutant, the peroxisomal methanol-pathway enzymes alcohol oxidase, catalase and dihydroxyacetone synthase were present and active but located in the cytosol. Together, the Pim-and Per-mutant collections involved mutations in 14 differentPERgenes. Two of the genes,PER5andPER7, were represented by both dominant-negative and recessive alleles. Diploids resulting from crosses of dominantperstrains and wild-typeH. polymorphawere Mut-and harbored peroxisomes with abnormal morphology. This is the first report of dominant-negative mutations affecting peroxisome biogenesis.