Tissue engineering of annulus fibrosus using electrospun fibrous scaffolds with aligned polycaprolactone fibers
Tissue engineering of annulus fibrosus using electrospun fibrous scaffolds with aligned polycaprolactone fibers
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DOI：
10.1002/jbm.a.33216
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发表时间：
2011-12-01
影响因子：
4.9
通讯作者：
Deng, Ying
中科院分区：
文献类型：
--
作者：
Koepsell, Laura;Remund, Tyler;Deng, Ying
在组织工程中，重要的是制造类似于天然组织的细胞外基质（ECM）和形貌外观的三维支架。本研究的目的是测试的假设，通过操纵纤维排列的相对程度不同的微观结构的静电纺丝纤维支架会影响猪纤维环细胞的行为。制备了五种具有无规或部分定向排列的聚己内酯纤维的静电纺丝纤维支架。已经检查了支架的微观结构，并且已经进行了体外实验以评估细胞-材料相互作用、细胞增殖和ECM产生。结果表明，具有定向纤维的支架对细胞定向和ECM分布提供了强有力的指导。此外，尽管电纺纤维支架的拉伸模量低于天然组织的拉伸模量，但它们与文献中报道的拉伸模量相当;因此，在包括支架材料选择和动态机械调节的优化条件下培养的构建体将具有更接近天然组织的模量的潜力。(C)2011 Wiley Periodicals，Inc. J Biomed Mater Res Part A：99A：564-575，2011.
In tissue engineering, it is important to fabricate a three-dimensional scaffold that resemble the extracellular matrix (ECM) and topographical appearance of native tissue. The aim of this study is to test the hypothesis that varying microstructures of electrospun fibrous scaffolds by manipulating the relative degree of fiber alignment would influence the behaviors of porcine annulus fibrosus cells. Five types of electrospun fibrous scaffolds with polycaprolactone fibers having random or partially aligned arrangements have been prepared and investigated. The scaffold microstructures have been examined, and in vitro experiments have been carried out to assess cell-material interaction, cell proliferation, and ECM production. The results indicate that the scaffold with oriented fibers provides strong guidance to the cell orientation and ECM distribution. In addition, albeit the tensile moduli of electrospun fibrous scaffolds are lower than that of native tissue, they are comparable to those reported in literature; hence, the constructs cultured with optimized conditions including the scaffold material selection and dynamic mechanical conditioning would have the potential to possess the moduli closer to that of native tissue. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 99A: 564-575, 2011.