Evidence for in vivo ribosome recycling, the fourth step in protein biosynthesis
Evidence for in vivo ribosome recycling, the fourth step in protein biosynthesis
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DOI：
10.1093/emboj/17.4.1141
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发表时间：
1998-02-16
期刊：
影响因子：
11.4
通讯作者：
Kaji, A
中科院分区：
文献类型：
--
作者：
Janosi, L;Mottagui-Tabar, S;Kaji, A
核糖体再循环因子（RRF）催化体外蛋白质合成的第四步：核糖体、mRNA和tRNA的终止后复合物的分解。我们现在报告的第一个在体内的证据RRF功能使用12个温度敏感的大肠杆菌突变体，我们在这项研究中分离。在非允许温度下，大多数核糖体保留在mRNA上，从终止密码子下游扫描，并在所有框架中的各个位点重新启动翻译，而不存在起始密码子。再起始不发生在终止密码子的上游，也不超过下游起始信号。RRF灭活在生长期是抑菌的，在稳定期和生长期之间的过渡期间是杀菌的，证实了体内蛋白质合成的第四步的本质。
Ribosome recycling factor (RRF) catalyzes the fourth step of protein synthesis in vitro: disassembly of the post-termination complex of ribosomes, mRNA and tRNA. We now report the first in vivo evidence of RRF function using 12 temperature-sensitive Escherichia coli mutants which we isolated in this study. At non-permissive temperatures, most of the ribosomes remain on mRNA, scan downstream from the termination codon, and re-initiate translation at various sites in all frames without the presence of an initiation codon. Reinitiation does not occur upstream from the termination codon nor beyond a downstream initiation signal. RRF inactivation was bacteriostatic in the growing phase and bactericidal during the transition between the stationary and growing phase, confirming the essential nature of the fourth step of protein synthesis in vivo.