Electricity production by Geobacter sulfurreducens attached to electrodes
Electricity production by Geobacter sulfurreducens attached to electrodes
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DOI：
10.1128/aem.69.3.1548-1555.2003
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发表时间：
2003-03-01
影响因子：
4.4
通讯作者：
Lovley, DR
中科院分区：
文献类型：
--
作者：
Bond, DR;Lovley, DR
以前的研究表明，土杆菌科的成员可以使用电极作为电子受体进行无氧呼吸。为了更好地理解这种用于能量生产的电子转移过程，将硫还原锗接种到其中石墨电极作为唯一电子受体且乙酸盐或氢作为电子供体的室中。通过将电子接受电极连接到含氧介质（燃料电池）中的类似电极或连接到相对于Ag/AgCl参比电极将电极保持在+0.2V（保持电位）的恒电位仪，将电子接受电极保持在氧化电位。当接种少量G.当将硫还原菌引入含电极的室中时，电流产生依赖于乙酸盐氧化成二氧化碳，并呈指数增加，首次表明电极还原支持该生物体的生长。当培养基被缺乏生长所需营养的厌氧缓冲液取代时，醋酸盐依赖的电流产生不受影响，附着在这些电极上的细胞继续产生电流数周。这代表了第一份关于微生物仅通过附着在电极上的细胞发电的报告。电极连接的细胞完全氧化醋酸盐至检测水平以下（
Previous studies have suggested that members of the Geobacteraceae can use electrodes as electron acceptors for anaerobic respiration. In order to better understand this electron transfer process for energy production, Geobacter sulfureducens was inoculated into chambers in which a graphite electrode served as the sole electron acceptor and acetate or hydrogen was the electron donor. The electron-accepting electrodes were maintained at oxidizing potentials by connecting them to similar electrodes in oxygenated medium (fuel cells) or to potentiostats that poised electrodes at +0.2 V versus an Ag/AgCI reference electrode (poised potential). When a small inoculum of G. sulfurreducens was introduced into electrode-containing chambers, electrical current production was dependent upon oxidation of acetate to carbon dioxide and increased exponentially, indicating for the first time that electrode reduction supported the growth of this organism. When the medium was replaced with an anaerobic buffer lacking nutrients required for growth, acetate-dependent electrical current production was unaffected and cells attached to these electrodes continued to generate electrical current for weeks. This represents the first report of microbial electricity production solely by cells attached to an electrode. Electrode-attached cells completely oxidized acetate to levels below detection (