Self-reinforcing injectable hydrogel with both high water content and mechanical strength for bone repair
Self-reinforcing injectable hydrogel with both high water content and mechanical strength for bone repair
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具有高含水量和机械强度的自增强可注射水凝胶，用于骨修复
DOI：
10.1016/j.cej.2015.12.021
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发表时间：
2016-03-15
影响因子：
15.1
通讯作者：
Liu, Mingzhu
中科院分区：
文献类型：
--
作者：
Bai, Xiao;Lu, Shaoyu;Liu, Mingzhu
在骨组织工程中构建水凝胶作为临时支架来支撑身体负荷的主要挑战是其机械强度差。通常在水凝胶中引入共价交联以增强其机械强度。然而，这种策略总是导致含水量的急剧减少。在这项研究中，一个自增强的可注射水凝胶的基础上非共价和Diels-Alder（DA）化学双重交联，以提高其机械强度和水含量。通过环糊精和金刚烷的超分子相互作用以及聚（N-异丙基丙烯酰胺）（PNIPAM）的溶胶-凝胶转变来设计非共价交联，从而在注射后原位形成水凝胶。通过糠胺接枝硫酸软骨素（ChS-F）和马来酰亚胺封端的聚乙二醇（PEG（2K）-AMI）发生DA化学交联，增加水凝胶的机械强度（E类似于25 MPa）。进行了体内骨修复试验，结果表明，实现了无细胞和无因子的纯水凝胶诱导的骨修复，证明了其在组织工程应用中的潜力。(C)2015爱思唯尔B. V.保留所有权利。
The main challenge for constructing hydrogels serving as a temporary skeleton to support body load in bone tissue engineering is its poor mechanical strength. Covalent crosslinking is generally introduced in hydrogel to enhance its mechanical strength. However, this strategy always results in comitant reduction in the water content. In this study, a self-reinforcing injectable hydrogel based on noncovalent and Diels-Alder (DA) chemical dual crosslinking is developed to improve both its mechanical strength and water content. The noncovalent crosslinking is designed through the supramolecular interaction of cyclodextrin and adamantane, and the sol-gel transition of poly(N-isopropyl acrylamide) (PNIPAM), enabling hydrogel formation in situ after injection. DA chemical crosslinking occurs via furfurylamine grafted chondroitin sulfate (ChS-F) and maleimido-terminated poly(ethylene glycol) (PEG(2K)-AMI), increasing the mechanical strength of hydrogel (E similar to 25 MPa). In vivo bone repair tests were performed, and the results indicated that a cell- and factor-free, purely hydrogel-induced bone repair is achieved, demonstrating its potential in tissue engineering applications. (C) 2015 Elsevier B.V. All rights reserved.