New insights into the genomic organization and splicing of the doublesex gene, a terminal regulator of sexual differentiation in the silkworm Bombyx mori.
New insights into the genomic organization and splicing of the doublesex gene, a terminal regulator of sexual differentiation in the silkworm Bombyx mori.
复制标题
对双性基因基因组组织和剪接的新见解，双性基因是家蚕性别分化的终端调节因子
DOI：
10.1371/journal.pone.0079703
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Xia Q
中科院分区：
文献类型：
--
作者：
Duan J;Xu H;Guo H;O'Brochta DA;Wang F;Ma S;Zhang L;Zha X;Zhao P;Xia Q
性别决定机制因生物而异。主要机制是多种多样的，而终端调节器是相对保守的。我们分析了家蚕双性基因（Bmdsx）的转录本，并报道了有关Bmdsx的基因组组织和表达的新结果。Bmdsx由9个外显子和8个内含子组成，其中2个外显子是新发现的，以前未见报道。Bmdsx转录本被剪接以产生17种可选剪接形式和11种推测的反式剪接变体。本文首次报道了13种交替剪接形式和5种推测的反式剪接形式。序列分析预测，在男性和女性中发现的10种女性特异性剪接形式、6种男性特异性剪接形式和1种剪接形式将产生4种女性特异性、2种男性特异性的Dsx蛋白和1种男性和女性共同的Dsx蛋白。Dsx蛋白有望发挥功能，调控下游靶基因。本文首次描述了一些预测的Dsx蛋白。因此，dsx基因在家蚕中的表达导致多种顺式和反式剪接转录物和多种dsx蛋白。这些发现表明，在家蚕中存在着复杂的dsx剪接模式，鳞翅目dsx的剪接调节和性别特异性功能也具有复杂性。
Sex-determination mechanisms differ among organisms. The primary mechanism is diverse, whereas the terminal regulator is relatively-conserved. We analyzed the transcripts of the Bombyx mori doublesex gene (Bmdsx), and reported novel results concerning the genomic organization and expression of Bmdsx. Bmdsx consists of nine exons and eight introns, of which two exons are novel and have not been reported previously. Bmdsx transcripts are spliced to generate seventeen alternatively-spliced forms and eleven putative trans-spliced variants. Thirteen of the alternatively-spliced forms and five of the putative trans-spliced forms are reported here for the first time. Sequence analysis predicts that ten female-specific, six male-specific splice forms and one splice form found in males and females will result in four female-specific, two male-specific Dsx proteins and one Dsx protein common to males and females. The Dsx proteins are expected to be functional and regulate downstream target genes. Some of the predicted Dsx proteins are described here for the first time. Therefore the expression of the dsx gene in B. mori results in a variety of cis- and trans-spliced transcripts and multiple Dsx proteins. These findings show that in B. mori there is a complicated pattern of dsx splicing, and that the regulation of splicing and sex-specific functions of lepidopteran dsx have evolved complexity.
登录
查看更多内容
影响因子：
3.4
作者：
Salvemini M;Mauro U;Lombardo F;Milano A;Zazzaro V;Arcà B;Polito LC;Saccone G
通讯作者：
Saccone G
影响因子：
5.3
作者：
SAMUELS, ME;BOPP, D;SCHEDL, P
通讯作者：
SCHEDL, P
影响因子：
5.3
作者：
SAMUELS, ME;SCHEDL, P;CLINE, TW
通讯作者：
CLINE, TW
影响因子：
4.5
作者：
Shao, Wei;Zhao, Qiong-Yi;Xu, Yong-Zhen
通讯作者：
Xu, Yong-Zhen
影响因子：
3.8
作者：
Shukla, J. N.;Nagaraju, J.
通讯作者：
Nagaraju, J.