Decreased miR-320 expression is associated with breast cancer progression, cell migration, and invasiveness via targeting Aquaporin 1
Decreased miR-320 expression is associated with breast cancer progression, cell migration, and invasiveness via targeting Aquaporin 1
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miR-320 表达减少通过靶向水通道蛋白 1 与乳腺癌进展、细胞迁移和侵袭性相关
DOI：
10.1093/abbs/gmy023
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发表时间：
2018-05-01
影响因子：
3.7
通讯作者：
Chen, Daozhen
中科院分区：
文献类型：
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作者：
Luo, Liang;Yang, Rui;Chen, Daozhen
我们以前的研究表明，水通道蛋白1（AQP 1）在乳腺癌中过表达。然而，这一机制仍然难以捉摸。MicroRNA 320（miR-320）下调已在各种类型的癌症中报道，它可能调节AQP 1的表达。在这项研究中，miR-320和AQP 1的表达进行了研究，通过定量逆转录-PCR，原位杂交和免疫组织化学。这些分子的临床病理意义进行了分析。我们发现miR-320在人乳腺癌患者的血浆和肿瘤组织中表达下调。生存分析显示miR-320表达减少和AQP 1过表达与预后不良相关。荧光素酶分析显示miR-320负调控AQP 1的表达。此外，进行细胞增殖、迁移和侵袭测定以研究miR-320对乳腺癌细胞的影响。我们的研究结果表明，miR-320过表达通过下调AQP 1抑制乳腺癌细胞的增殖、迁移和侵袭。这些观察结果表明，miR-320下调可能会增强乳腺癌中AQP 1的表达，有利于肿瘤进展。我们的研究结果表明，miR-320和AQP 1可能作为乳腺癌治疗的预后生物标志物和治疗靶点。
Our previous studies have demonstrated that Aquaporin 1 (AQP1) is overexpressed in breast cancer. However, the mechanism remains elusive. MicroRNA 320 (miR-320) downregulation has been reported in various types of cancers, and it may regulate AQP1 expression. In this study, miR-320 and AQP1 expressions were investigated by quantitative reverse transcription-PCR, in situ hybridization, and immunohistochemistry. The clinicopathological implications of these molecules were also analyzed. We found that miR-320 expression is downregulated in both plasma and tumor tissue in human breast cancer patients. Survival analysis showed that reduced expression of miR-320 and overexpression of AQP1 are associated with worse prognosis. Luciferase assays showed that miR-320 negatively regulates AQP1 expression. In addition, cell proliferation, migration, and invasion assays were performed to investigate the effects of miR-320 on breast cancer cells. Our results showed that miR-320 overexpression inhibits cell proliferation, migration, and invasion in breast cancer cells by downregulating AQP1. These observations suggested that miR-320 downregulation may enhance AQP1 expression in breast cancer, favoring tumor progression. Our findings indicated that miR-320 and AQP1 may serve as prognostic biomarkers and therapeutic targets in the treatment of breast cancer.