Enhancement of BODIPY505/515 lipid fluorescence method for applications in biofuel-directed microalgae production
Enhancement of BODIPY505/515 lipid fluorescence method for applications in biofuel-directed microalgae production
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DOI：
10.1016/j.mimet.2012.03.020
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发表时间：
2012-08-01
影响因子：
2.2
通讯作者：
Owende, Philip
中科院分区：
文献类型：
--
作者：
Brennan, Liam;Fernandez, Alfonso Blanco;Owende, Philip
本文介绍了一种基于BODIPY 505/515的微藻细胞脂质荧光增强方法，该方法可用于筛选野生型微藻中的脂质，并监测微藻生产过程中的脂质含量，以确定最佳收获时间。这项研究是基于四种微藻物种（杜氏藻，Tetraselmis suecica，Nannochloropsis oculata和Nannochloris atomus），因为它们固有的高脂质含量。用N.由于与其他实验菌株相比，观察到的荧光降低，因此，针对两种溶剂预处理方法（DMSO和甘油）和四种染色条件参数（分析时间、染色温度、染料浓度和藻细胞浓度）测定BODIPY 505/515脂质荧光。结果表明，厚细胞壁微藻如N。通过预处理方法和染色条件参数显著增强，从而通过CA显著增强脂质荧光。800倍的基础自发荧光。脂质荧光增强方法为总脂质含量的体内流式细胞术定量提供了快速和简单的指标，用于生物燃料定向微藻生产中的物种筛选或全培养物监测。(C)2012爱思唯尔有限公司版权所有。
This paper describes a microalgal cell lipid fluorescence enhancement method using BODIPY505/515, which can be used to screen for lipids in wild-type microalgae and to monitor lipid content within microalgae production processes to determine optimal harvesting time. The study was based on four microalgae species (Dunaliella teteriolecta, Tetraselmis suecica, Nannochloropsis oculata, and Nannochloris atomus) selected because of their inherent high lipid content. An extended analysis was carried out with N. oculata due to the depressed fluorescence observed when compared with the other experimental strains. BODIPY505/515 lipid fluorescence was determined for two solvent pre-treatment methods (DMSO and glycerol) and four staining condition parameters (analysis time, staining temperature, dye concentration, and algal cell concentration). It was found that lipid fluorescence of thick cell-walled microalgae, such as N. oculata, is significantly enhanced by both the pre-treatment methods and staining condition parameters, thereby significantly enhancing lipid fluorescence by ca. 800 times the base autofluorescence. The lipid fluorescence enhancement method provides a quick and simple index for in vivo Flow Cytometry quantification of total lipid contents for purposes of species screening or whole culture monitoring in biofuel-directed microalgae production. (C) 2012 Elsevier B.V. All rights reserved.