Contribution of neural cell adhesion molecule (NCAM) to hemopoietic system in monkeys
Contribution of neural cell adhesion molecule (NCAM) to hemopoietic system in monkeys
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DOI：
10.1007/s00277-008-0513-9
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发表时间：
2008-10-01
影响因子：
3.5
通讯作者：
Ikehara, Susumu
中科院分区：
文献类型：
--
作者：
Kato, Junko;Hisha, Hiroko;Ikehara, Susumu
神经细胞粘附分子（Neural cell adhesion molecules，CD 56）是一种重要的粘附分子，主要表达于神经细胞和自然杀伤细胞。虽然新鲜分离的食蟹猴骨髓细胞（BMC）仅含有少量CD 56阳性细胞，但几乎所有BM贴壁细胞（培养2- 3周后获得的BMC）均用抗CD 56单克隆抗体（mAb）染色呈阳性。BM贴壁细胞显示均匀的成纤维细胞形态，血淋巴样标志物（CD 4、CD 8、CD 11b、CD 14、CD 34和CD 45）阴性。在诱导培养条件下观察成脂和成骨。骨髓贴壁细胞在体外具有支持造血干细胞（HSCs）造血的能力，加入抗CD 56单克隆抗体可明显抑制HSCs的增殖。CD 56分子也在HSC上表达，因为约20%的HSC富集群体（谱系阴性和胚细胞门控细胞）对CD 56呈阳性。此外，猴骨髓切片的免疫染色显示，许多基质细胞是CD 56阳性的，并且一些CD 56阳性基质细胞与CD 56阳性造血细胞直接接触。这些结果表明，CD 56分子在猴的HSC和BM基质细胞（包含MSC）上均表达，因此可以推测，CD 56也有助于人类造血系统。
Neural cell adhesion molecules (CD56) are important adhesion molecules that are mainly expressed on neural cells and natural killer cells. Although freshly isolated cynomolgus monkey bone marrow cells (BMCs) contained only a few CD56-positive cells, almost all the BM adherent cells (obtained after a 2- to 3-week culture of the BMCs) were stained positively with anti-CD56 monoclonal antibody (mAb). The BM adherent cells showed uniformly fibroblastic morphology and were negative for hematolymphoid markers (CD4, CD8, CD11b, CD14, CD34, and CD45). Adipogenesis and osteogenesis were observed under inductive culture conditions. The BM adherent cells had the ability to support hemopoiesis of hemopoietic stem cells (HSCs) in vitro, and the proliferation of HSCs was significantly inhibited by the addition of anti-CD56 mAb to the coculture system. CD56 molecules were also expressed on HSCs because about 20% of an HSC-enriched population (lineage-negative and blast-gated cells) was positive for CD56. In addition, the immunostaining of monkey BM sections revealed that many stromal cells were CD56-positive, and some CD56-positive stromal cells came into direct contact with CD56-positive hemopoietic cells. These results indicate that the CD56 molecule is expressed on both HSCs and BM stromal cells (containing MSCs) in monkeys, and therefore it can be speculated that CD56 also contributes to the human hematopoietic system.