Biphasic change of progenitor proliferation in dentate gyrus after single dose of isoflurane in young adult rats.
Biphasic change of progenitor proliferation in dentate gyrus after single dose of isoflurane in young adult rats.
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DOI：
10.1097/ana.0b013e318283c3c7
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发表时间：
2013-07
影响因子：
3.7
通讯作者：
Sall JW
中科院分区：
文献类型：
--
作者：
Lin N;Moon TS;Stratmann G;Sall JW
异氟烷暴露可改善年轻成年大鼠的长期神经认知功能，这与麻醉后4天齿状回祖细胞增殖增加有关。然而，麻醉后4天从掺入溴脱氧尿苷的细胞中产生的新神经元的数量不受麻醉的影响。我们测试了祖细胞增殖在过去4天继续增加的假设，这意味着如果溴脱氧尿苷标记发生在稍后的时间点，麻醉后新神经元的数量可能会增加。60日龄大鼠吸入异氟醚4 h后，分别于0、1、2、4、9、16天注射溴脱氧尿苷。2小时后收集脑，化学染色，并在显微镜下评估齿状回中溴脱氧尿苷阳性细胞的数量。在60日龄大鼠中暴露于异氟烷4小时后，溴脱氧尿苷阳性细胞的数量在第0-2天减少，然后在第4天显著增加，并在第9天和第16天回归到对照水平。麻醉诱导的祖细胞增殖在齿状回麻醉后9天没有持续。我们解释这些结果表明，麻醉对神经发生的影响可能不会发挥关键作用，在以前观察到的异氟烷诱导的长期改善神经认知功能的60天龄大鼠和祖细胞增殖的短暂增加，以补充池的神经干细胞。麻醉诱导的年轻成年大鼠认知能力改善的机制仍然不清楚。
Isoflurane exposure causes improvement in long-term neurocognitive function in young adult rats, this is associated with an increase in dentate gyrus progenitor proliferation 4 days after anesthesia. However, the number of new neurons, that were born from cells that incorporated bromodeoxyuridine 4 days after anesthesia is not affected by anesthesia. We tested the hypothesis that progenitor proliferation continues to increase past 4 days, which would imply the possibility that the number of new neurons after anesthesia could be increased if bromodeoxyuridine labeling occurred at a later time point. Bromodeoxyuridine was injected at 0, 1, 2, 4, 9, 16 days after 4 hours of isoflurane exposure to 60-day old rats. Brains were harvested 2 hours later, immunohistochemically stained, and the number of bromodeoxyuridine-positive cells in the dentate gyrus was assessed microscopically. After 4 hours of exposure to isoflurane in 60-day old rats, the number of bromodeoxyuridine-positive cells decreased on days 0–2, then increased on day 4 significantly, and regressed toward the control level on day 9 and 16. Anesthesia-induced progenitor proliferation in the dentate gyrus was not sustained 9 days after anesthesia. We interpret these results to signify that an anesthetic effect on neurogenesis likely does not play a critical role in the previously observed isoflurane-induced long-term improvement in neurocognitive function in 60-day old rats and that the transient increase in progenitor proliferation serves to replenish the pool of neural stem cells. The mechanism of anesthesia-induced improvement in cognition of young adult rats remains elusive.