The Embryonic Expression Patterns and the Knockdown Phenotypes of Zebrafish ADP-Ribosylation Factor-Like 6 Interacting Protein Gene
The Embryonic Expression Patterns and the Knockdown Phenotypes of Zebrafish ADP-Ribosylation Factor-Like 6 Interacting Protein Gene
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DOI：
10.1002/dvdy.21832
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发表时间：
2009-01-01
影响因子：
2.5
通讯作者：
Tsai, Huai-Jen
中科院分区：
文献类型：
--
作者：
Huang, Hsing-Yen;Dai, En-Sheng;Tsai, Huai-Jen
ADP-核糖基化因子样6（Arl 6）突变与人类疾病相关，并且Arl 6与Arl 6相互作用蛋白（Arl 6 ip）相互作用。然而，Arl 6 ip在胚胎发生过程中的表达模式和功能尚不清楚。以确认Arl 6 ip功能是否异常。可能;结果在斑马鱼胚胎发育过程中，我们检测了ARL 6 IP的表达模式，它们在脑、视原基、下索、脊髓、肌节、心脏、鳍芽、肾脏、躯干和视网膜中呈母系表达和展示。Arl 6 ip的敲除揭示了以下表型缺陷：小眼症、色素模式紊乱、平头、顶盖缺陷、胸鳍缺陷、气道异常、心包水肿和躯干变形。特别地，ar 16 ip-吗啡变体的视网膜的组织学解剖揭示了神经元分化严重延迟，导致没有视网膜层的形成。我们进一步证实ar 16 ip-morphants的视蛋白不被转录。基于这一证据，Arl 6 ip可能在斑马鱼的眼，心脏和鳍芽发育中发挥重要作用。发展动力学238：232-240，2009年。(C)2008威利利斯公司
ADP-ribosylation factor-like 6 (Arl6) mutation is linked to human disease and Arl6 interacts with Arl6 interacting protein (Arl6ip). However, the expression pattern and function of Arl6ip during embryogenesis are unknown. To confirm whether abnormal Arl6ip function. might; result in embryonic defects in zebrafish, we examined the expression patterns of arl6ip during embryogenesis, and they were maternally expressed and exhibited in the brain, optic primordia, hypochord, spinal cord, myotome, heart, fin-bud, kidney, trunk, and retina. Knockdown of Arl6ip revealed the following phenotypic defects: microphthalmia, disorganized pigment pattern, flat head, defective tectum, deficient pectoral fins, abnormal pneumatic duct, pericardial edema, and deformed trunk. Particularly, histological dissection of the retinae of arl6ip-morphants revealed that neuronal differentiation is severely delayed, resulting in no formation of retinal layers. We further confirmed that opsins of arl6ip-morphants were not transcribed. Based on this evidence, Arl6ip may play important roles in zebrafish ocular, heart, and fin-bud development. Developmental Dynamics 238: 232-240, 2009. (C) 2008 Wiley-Liss, Inc.