Influence of solvent uptake and swelling by poly(styrene-divinylbenzene) column packings on sample sorption rate and band broadening in reversed-phase liquid chromatography
Influence of solvent uptake and swelling by poly(styrene-divinylbenzene) column packings on sample sorption rate and band broadening in reversed-phase liquid chromatography
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DOI：
10.1016/s0021-9673(98)01070-x
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发表时间：
1999-03-12
影响因子：
4.1
通讯作者：
Cantwell, FF
中科院分区：
文献类型：
--
作者：
Ells, B;Wang, Y;Cantwell, FF
多孔聚（苯乙烯-二乙烯基苯）（PS-DVB）HPLC填料对某些类型的溶质产生宽尾峰。这种现象是由于这些溶质吸附到聚合物基质中，在那里它们经历非常缓慢的受阻扩散。众所周知，甲醇（MeOH）-H2O移动的相中存在少量四氢呋喃（THF）会产生此类溶质的较窄且尾部较少的峰。在这项研究中，汉密尔顿PRP无限，无孔PS-DVB聚合物，作为多孔聚合物基质的模型。进行了以下测量：从水溶液中的MeOH和THF的吸附等温线; PRP-无限的溶胀作为在水溶液中的MeOH和THF的活性的函数;以及从各种溶剂混合物中的溶质萘的吸附等温线和吸附速率曲线。添加2%THF到70：30 MeOH-H2O中产生额外的0.4%溶胀，吸附容量降低11%，萘在PRP-infinity中的扩散系数增加90%。扩散系数的增加，这是负责萘的改进的洗脱峰形状，被证明是由吸附能力的降低，而不是由额外的溶胀。吸附的THF用于填充和“阻塞”聚合物基质中最小、最高阻碍的微孔。(C)1999 Elsevier Science B. V.保留所有权利。
Porous poly(styrene-divinylbenzene) (PS-DVB) HPLC packings give broad, tailed peaks for some types of solutes. This phenomenon is due to sorption of these solutes into the polymer matrix where they experience very slow, hindered diffusion. It is known that the presence of small amounts of tetrahydrofuran (THF) in the methanol (MeOH)-H2O mobile phase yields narrower and less tailed peaks for such solutes. In this study, Hamilton PRP-infinity, a nonporous PS-DVB polymer, serves as a model for the matrix of porous polymers. The following measurements were made: sorption isotherms for MeOH and THF from aqueous solution; swelling of PRP-infinity as a function of activity of MeOH and THF in aqueous solution; and both sorption isotherms and sorption rate curves for the solute naphthalene from various solvent mixtures. The addition of 2% THF to 70:30 MeOH-H2O produced an additional 0.4% swelling, an 11% decrease in the sorption capacity and a 90% increase in the diffusion coefficient of naphthalene in PRP-infinity. The increase in the diffusion coefficient, which is responsible for the improved elution peak shape of naphthalene, is shown to be caused by the decrease in sorption capacity, rather than by the additional swelling. The sorbed THF serves to fill and "block" the smallest, most highly hindered micropores in the polymer matrix. (C) 1999 Elsevier Science B.V. All rights reserved.