Changes of glucocorticoid receptor and levels of CRF mRNA, POMC mRNA in brain of chronic immobilization stress rats
Changes of glucocorticoid receptor and levels of CRF mRNA, POMC mRNA in brain of chronic immobilization stress rats
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DOI：
10.1007/s10571-007-9170-0
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发表时间：
2008-02-01
影响因子：
4
通讯作者：
Yang, Jian-Xin
中科院分区：
文献类型：
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作者：
Chen, Jia-Xu;Tang, Yi-Ting;Yang, Jian-Xin
慢性制动应激（CIS）对生化指标的影响一直是神经科学研究的热点之一。本研究旨在探讨CIS对大鼠脑组织糖皮质激素受体（GR）、促肾上腺皮质激素释放因子（CRF）和阿黑皮素原（POMC）mRNA水平的影响。将大鼠随机分为应激组和对照组。应激组给予CIS 3 h/d，连续21 d。采用免疫组织化学方法检测大鼠海马和前额叶皮质GR的表达。采用逆转录-聚合酶链反应（RT-PCR）检测大鼠下丘脑、垂体、海马、PFC等脑区CRFmRNA和POMC mRNA的表达。CIS暴露21天后，应激组海马CA 1区、齿状回、PFC区GR免疫染色（灰度值）均低于对照组（P < 0.01）。定量分析显示，应激组大鼠下丘脑和垂体中有CRF mRNA表达，而前额叶、海马和垂体中POMC mRNA表达较对照组减少（P < 0.01）。不同脑区GR、CRFmRNA和POMC mRNA水平的降低可能有助于解释CIS的作用机制。
The effect of chronic immobilization stress (CIS) on the biochemical parameters has been one of the hot topics in neuroscience. The aim of this study was to investigate the effects of CIS on the levels of glucocorticoid receptor (GR), corticotrophin-releasing factor (CRF) mRNA and proopiomelanocortin (POMC) mRNA in brains of rats. The rats were randomly divided into stressed and control groups. The stressed group was given CIS 3 h a day for 21 days continuously. GR of rats' hippocampus and prefrontal cortex (PFC) were detected by immunohistochemistry method. In addition, the CRF mRNA and POMC mRNA of rats' brain regions (hypothalamus, pituitary, hippocampus, and PFC) were detected by reverse transcription-polymerase chain reaction (RT-PCR). After exposure to CIS for 21 days, the GR immuno staining (the gray values) of the stressed group was less than that of the control group in hippocampal CA(1), dentate gyrus, and PFC (P < 0.01). Quantitative analysis indicated the presence of CRF mRNA in hypothalamus and pituitary, while POMC mRNA in PFC, hippocampus and pituitary of the stressed group was less than that of the control group (P < 0.01). The decreased levels of GR, CRF mRNA, and POMC mRNA in different brain regions may contribute to explanation of the CIS induced mechanism.