Pyrosequencing-Based Assessment of Soil pH as a Predictor of Soil Bacterial Community Structure at the Continental Scale
Pyrosequencing-Based Assessment of Soil pH as a Predictor of Soil Bacterial Community Structure at the Continental Scale
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DOI：
10.1128/aem.00335-09
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发表时间：
2009-08-01
影响因子：
4.4
通讯作者：
Fierer, Noah
中科院分区：
文献类型：
--
作者：
Lauber, Christian L.;Hamady, Micah;Fierer, Noah
土壤中有各种各样的细菌种群，土壤细菌群落的组成在空间上可能有很大差异。然而，我们对土壤细菌群落结构在较大空间尺度上发生的具体变化的理解是有限的，因为大多数以前的工作都集中在详细调查相对较少的土壤或分析大量的土壤与技术，提供有关细菌群落的系统发育结构的细节很少。在这里，我们使用条形码焦磷酸测序技术来表征来自北美和南美的88种土壤中的细菌群落，平均每种土壤获得1，501个序列。我们发现，整体细菌群落组成，成对UniFrac距离测量，显着相关的土壤pH值的差异（r = 0.79），主要是由变化的相对丰度的酸杆菌，放线菌，拟杆菌在整个土壤pH值的范围。此外，土壤pH值解释了一个显着的变异与观察到的变化，在每个优势谱系内的系统发育结构。细菌群落的总体系统发育多样性也与土壤pH相关（R-2 = 0.50），在近中性pH的土壤中具有峰值多样性。总之，这些结果表明，土壤细菌群落的结构是可预测的，在一定程度上，在更大的空间尺度，土壤pH值对细菌群落组成的影响是显而易见的，即使在相对粗糙的水平的分类分辨率。
Soils harbor enormously diverse bacterial populations, and soil bacterial communities can vary greatly in composition across space. However, our understanding of the specific changes in soil bacterial community structure that occur across larger spatial scales is limited because most previous work has focused on either surveying a relatively small number of soils in detail or analyzing a larger number of soils with techniques that provide little detail about the phylogenetic structure of the bacterial communities. Here we used a bar-coded pyrosequencing technique to characterize bacterial communities in 88 soils from across North and South America, obtaining an average of 1,501 sequences per soil. We found that overall bacterial community composition, as measured by pairwise UniFrac distances, was significantly correlated with differences in soil pH (r = 0.79), largely driven by changes in the relative abundances of Acidobacteria, Actinobacteria, and Bacteroidetes across the range of soil pHs. In addition, soil pH explains a significant portion of the variability associated with observed changes in the phylogenetic structure within each dominant lineage. The overall phylogenetic diversity of the bacterial communities was also correlated with soil pH (R-2 = 0.50), with peak diversity in soils with near-neutral pHs. Together, these results suggest that the structure of soil bacterial communities is predictable, to some degree, across larger spatial scales, and the effect of soil pH on bacterial community composition is evident at even relatively coarse levels of taxonomic resolution.