Isolation and Characterization of Pseudomonas Strains Capable of Fe(III) Reduction with Reference to Redox Response Regulator Genes
Isolation and Characterization of Pseudomonas Strains Capable of Fe(III) Reduction with Reference to Redox Response Regulator Genes
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DOI：
10.1080/01490450600596565
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发表时间：
2006-07
影响因子：
2.3
通讯作者：
T. Naganuma;Makoto Sato;Daiki Hoshii;Yuki Amano-Murakami;T. Iwatsuki;K. Mandernack
中科院分区：
文献类型：
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作者：
T. Naganuma;Makoto Sato;Daiki Hoshii;Yuki Amano-Murakami;T. Iwatsuki;K. Mandernack
从日本中部岐阜县东野铀矿地下水中分离出两种细菌菌株，KNA-6-3和KNA-6-5，它们表现出轻微但持久的三价铁（Fe（III））还原。菌株的16 S rDNA序列与兼性细菌施氏假单胞菌（Pseudomonasstutzeri）的相似性> 96%，施氏假单胞菌是一种尚未被认为是Fe（III）还原剂的物种。利用PCR技术扩增了参与有氧代谢和无氧代谢转换的氧化还原应激反应调节因子arcA、arcB和anr基因，并进行了序列测定。系统发育分析表明，扩增序列与已知的arcA，arcB和anr基因从其他细菌物种的高度相似。在有氧和厌氧生长过程中，通过实时逆转录PCR监测推定的arcA，arcB和anr基因的表达。假定的arcA和anr的成绩单增加轻微但持久的Fe（III）的减少，而假定的arcB成绩单没有表现出显着的相关性与Fe（III）的减少。来自两种菌株的推定anr序列不太相似，因此推定arcA序列被用于估计Tono铀矿地下水中相关细菌的丰度。使用通用arcA引物和arcA探针进行的实时基因组PCR显示，arcA携带者在8.05 × 104个细胞/ml的总微生物种群中的比例低至0.06%（50个细胞/ml）。
Two bacterial strains, KNA-6-3 and KNA-6-5, were isolated from groundwater of the Tono uranium mine, Gifu Prefecture, central Japan, and showed slight but persistent ferric iron (Fe(III)) reduction. The 16S rDNA sequences of the strains were > 96% similar to that of the facultative denitrifier Pseudomonas stutzeri, a species that has not been regarded as a reducer of Fe(III). The genes of redox stress response regulators involved in switching between aerobic and anaerobic metabolisms, arcA, arcB and anr, were amplified by PCR and sequenced. Phylogenetic analyses of the amplified sequences showed high similarities to known arcA, arcB and anr genes from other bacterial species. Expression of the putative arcA, arcB and anr genes was monitored by real-time reverse transcription PCR during aerobic and anaerobic growth. Transcripts of the putative arcA and anr increased with slight but persistent Fe(III) reduction, whereas the putative arcB transcript showed no significant correlation with Fe(III) reduction. The putative anr sequences from the two strains were less similar, and therefore the putative arcA sequences were targeted to estimate the abundance of related bacteria in the Tono uranium mine groundwater. Real-time genomic PCR using universal arcA primers and an arcA probe showed that arcA-bearers would occur at as low as 0.06% (50 cells ml−1) of the total microbial population of 8.05 × 104 cells ml−1.