Polymorphisms of the prion protein gene and their effects on litter size and risk evaluation for scrapie in Chinese Hu sheep.
Polymorphisms of the prion protein gene and their effects on litter size and risk evaluation for scrapie in Chinese Hu sheep.
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中国湖羊朊病毒蛋白基因多态性及其对产羔数的影响及痒病风险评估
DOI：
10.1007/s11262-011-0609-5
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发表时间：
2011-08
期刊：
影响因子：
1.6
通讯作者：
Shi, Guoqing
中科院分区：
文献类型：
--
作者：
Guan, Feng;Pan, Lei;Li, Jie;Tang, Hong;Zhu, Cheng;Shi, Guoqing
羊瘙痒病是绵羊和山羊的一种致死性神经退行性疾病，属于传染性海绵状脑病（transmissible spongiform encephalopathies，TSE）或朊病毒病。朊蛋白基因（PRNP）密码子136、154和171位点的多态性与羊瘙痒病的发生密切相关。本研究在180只湖羊中检测到PRNP基因密码子136、154、171和其他6个位点（密码子101、112、127、137、138、152）的9个多态性。密码子136、154和171处的所有等位基因已被鉴定，并导致三种新的基因型。A136、R154和Q171的优势等位基因频率分别为85%、99.40%和37.78%。优势单倍型ARQ的频率较高，为57.77%。ARR/ARQ和ARQ/ARQ的优势基因型频率分别为30%和26.67%。ARQ/TRK、ARQ/TRR和TRR/TRQ三种新发现的基因型频率均较低（0.56%）。PRNP基因型与羊瘙痒病发病风险和产羔数的相关分析表明，R1、R2和R3基因型分别占优势基因型的1.67%、32.22%和42.22%。在第一胎时，ARH/ARH基因型个体的产仔数显著高于平均值和ARQ/ARQ、ARR/ARQ基因型个体。本研究为中国湖羊PRNP基因的等位基因和基因型研究提供了初步资料。因此，湖羊对自然痒病的易感性较低，优势PRNP基因型至少对产羔数没有显著影响。
It is well known that scrapie is a fatal, neurodegenerative disease in sheep and goat, which belongs to the group of transmissible spongiform encephalopathies (TSEs) or prion diseases. It has been confirmed that the polymorphisms of prion protein gene (PRNP) at codons 136, 154, and 171 have strong relationship with scrapie in sheep. In the present study, nine polymorphisms of PRNP at codons 136, 154, and 171 and other six loci (at codons 101, 112, 127, 137, 138, and 152) were detected in 180 Chinese Hu sheep. All the alleles at codons 136, 154, and 171 have been identified and resulted in three new genotypes. The frequencies of predominant alleles were 85% (A136), 99.40% (R154), and 37.78% (Q171), respectively. The predominant haplotype ARQ has a relatively high frequency of 57.77%. The frequencies of dominant genotypes of ARR/ARQ and ARQ/ARQ were 30 and 26.67%, respectively. Three new found genotypes named ARQ/TRK, ARQ/TRR, and TRR/TRQ had the same lower frequencies (0.56%). The relationship of PRNP genotype with scrapie risk and litter size showed that the predominant genotypes are corresponded to the risk score of R1(1.67%), R2(32.22%), and R3(42.22%). Just at the first parity, the individuals with ARH/ARH genotype had significantly larger litter size than the mean value and those with ARQ/ARQ and ARR/ARQ genotypes. In short, this study provided preliminary information about alleles and genotypes of PRNP in Chinese Hu sheep. It could be concluded that Hu sheep has a low susceptibility to natural scrapie, and the predominant PRNP genotype at least has no significant effect on litter size.
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影响因子：
4
作者：
Goldmann, W;Baylis, M;Hunter, N
通讯作者：
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作者：
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作者：
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