Involvement of phospholipase PLA(2) in production of cellulase and xylanase by Penicillium oxalicum
Involvement of phospholipase PLA(2) in production of cellulase and xylanase by Penicillium oxalicum
复制标题
磷脂酶 PLA2 参与草酸青霉生产纤维素酶和木聚糖酶
DOI：
10.1007/s00253-020-11065-1
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发表时间：
2021
影响因子：
5
通讯作者：
Jia-Xun Feng
中科院分区：
文献类型：
--
作者：
Shi-Huan Li;Li-Sha Gu;Xiao-Yi Qu;Ting Zhang;Cheng-Xi Li;Rong-Ming Mai;Lu-Sheng Liao;Feng-Fei Zhang;Xue-Mei Luo;Shuai Zhao;Jia-Xun Feng
摘要磷脂酶在哺乳动物和植物的免疫和炎症反应中起着重要作用，然而，对真菌中磷脂酶功能的了解有限。在本研究中，我们研究了预测的磷脂酶基因删除纤维素酶和木聚糖酶的生产，以及形态表型的影响，在青霉。10个预测的磷脂酶基因中有9个基因的缺失导致纤维素酶和木聚糖酶的产量以及形态表型发生不同程度的改变。突变体P07277损失了22.5%至82.8%的纤维素酶（即，滤纸纤维素酶、羧甲基纤维素酶和对硝基苯基-β-纤维二糖酶）和木聚糖酶的产量，其中对硝基苯基-β-吡喃葡萄糖苷酶的产量增加了5.8-127.8倍。[咒语]比较转录组学和定量逆转录PCR分析表明，POX 07277对纤维素酶和木聚糖酶基因以及真菌产孢调控基因的表达具有动态影响，POX 07277对已知的调控基因PoxCxr B（纤维素分解和木聚糖分解调控因子B）的表达具有调控作用。该基因与P. [咒语]相反，POX 07277的表达受PoxCxrB的调控。这些发现将有助于理解磷脂酶的功能，并为真菌纤维素酶和木聚糖酶基因表达机制提供新的见解。关键点·研究了磷脂酶在青霉中的作用。·POX 07277（PLA 2）是纤维素酶和木聚糖酶基因表达所必需的。PoxCxrB动态调节POX 07277的表达。
AbstractPhospholipases play vital roles in immune and inflammatory responses in mammals and plants; however, knowledge of phospholipase functions in fungi is limited. In this study, we investigated the effects of deleting predicted phospholipase genes on cellulase and xylanase production, and morphological phenotype, inPenicillium oxalicum. Individual deletion of nine of the ten predicted phospholipase genes resulted in alteration of cellulase and xylanase production, and the morphological phenotypes, to various degrees. The mutant ∆POX07277lost 22.5 to 82.8% of cellulase (i.e., filter paper cellulase, carboxymethylcellulase, andp-nitrophenyl-β-cellobiosidase) and xylanase production, whereasp-nitrophenyl-β-glucopyranosidase production increased by 5.8–127.8 fold.POX07277(P. oxalicumgene No. 07277) was predicted to encode phospholipase A2 and was found to negatively affect the sporulation ofP. oxalicum. Comparative transcriptomic and quantitative reverse transcription-PCR analysis indicated thatPOX07277dynamically affected the expression of cellulase and xylanase genes and the regulatory genes for fungal sporulation, under micro-crystalline cellulose induction.POX07277was required for the expression of the known regulatory genePoxCxrB(cellulolytic and xylanolytic regulator B inP. oxalicum), which is involved in cellulase and xylanase gene expression inP. oxalicum. Conversely,POX07277expression was regulated byPoxCxrB. These findings will aid the understanding of phospholipase functions and provide novel insights into the mechanism of fungal cellulase and xylanase gene expression.Key points• The roles of phospholipases were investigated in Penicillium oxalicum.• POX07277 (PLA2) is required for the expression of cellulase and xylanase genes.• PoxCxrB dynamically regulated POX07277 expression.