Evaluation of third-generation RIDASCREEN enzyme immunoassay for the detection of norovirus antigens in stool samples of hospitalized children in Belem, Para, Brazil
Evaluation of third-generation RIDASCREEN enzyme immunoassay for the detection of norovirus antigens in stool samples of hospitalized children in Belem, Para, Brazil
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DOI：
10.1016/j.diagmicrobio.2011.08.023
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发表时间：
2011-12-01
影响因子：
2.9
通讯作者：
Gabbay, Yvone Benchimol
中科院分区：
文献类型：
--
作者：
Monteiro Siqueira, Jones Anderson;Linhares, Alexandre da Costa;Gabbay, Yvone Benchimol
诺如病毒（No v）是全世界胃肠炎暴发和住院治疗的主要病原体。本研究评估了市售的第三代RIDASCREEN (R)诺如病毒酶免疫测定（EIA）试剂盒与逆转录聚合酶链反应（RT-PCR）检测肠胃炎住院儿童NoVs的敏感性和特异性。将EIA与RT-PCR进行比较，一致性为88%（81/92）。灵敏度为92%，特异性为83.3%。11份样品仅用1种方法检测阳性（4种RT-PCR/7种EIA）。对14份样品进行测序，均归类为NoV基因组GII-4。电镜观察结果显示，3份（42.9%）的病毒颗粒表现为新型病毒的形态。半嵌套rt - pcr检测结果为85.7%。在这项研究中获得的结果表明，在敏感性和特异性的显著改善，这种更新的分析。(C) 2011爱思唯尔公司版权所有。
Noroviruses (No Vs) are major agents of gastroenteritis outbreaks and hospitalization worldwide. This study evaluated the sensitivity and specificity of the commercially available third-generation RIDASCREEN (R) Norovirus Enzyme Immunoassay (EIA) kit in comparison to the reverse transcription-polymerase chain reaction (RT-PCR) to detect NoVs in hospitalized children with gastroenteritis. An agreement of 88% (81/92) was observed when comparing EIA with RT-PCR. A sensitivity of 92% and a specificity of 83.3% were demonstrated. Eleven samples were positive by 1 method only (4 RT-PCR/7 EIA). Fourteen samples were sequenced and all classified as NoV genogroup GII-4. The 7 positive only by EIA were also evaluated by electron microscopy, and in 3 (42.9%) samples viral particles with a suggestive morphology of NoVs were visualized. These same samples were tested by seminested-RT-PCR with a positivity of 85.7%. The results obtained in this study demonstrated a significant improvement in the sensitivity and specificity of this updated assay. (C) 2011 Elsevier Inc. All rights reserved.