Preferential binding of vasoactive intestinal polypeptide to basolateral membrane of rat and rabbit enterocytes.
Preferential binding of vasoactive intestinal polypeptide to basolateral membrane of rat and rabbit enterocytes.
复制标题
血管活性肠多肽优先结合大鼠和兔肠细胞的基底外侧膜。
DOI：
10.1172/jci110748
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Wright,EM
中科院分区：
文献类型：
--
作者：
Dharmsathaphorn,K;Harms,V;Yamashiro,DJ;Hughes,RJ;Binder,HJ;Wright,EM
本文研究了放射性碘标记血管活性肠肽（VIP）与兔回肠和大鼠空肠肠细胞膜的结合。125 I标记的VIP的特异性结合，可以证明只有在基底侧膜上，而不是刷状缘膜。这与VIP应用于兔回肠体外制备的粘膜侧时对离子转运缺乏影响相对应。VIP改变离子转运只有当它被施加到serenum侧。~（125）I-VIP的结合是特异性的，并依赖于温育温度。VIP对125 I-VIP结合的抑制作用与其对腺苷酸环化酶活性的增强作用密切相关。这些观察结果表明，VIP受体位于基底外侧膜上。
Binding of radioiodinated vasoactive intestinal polypeptide (VIP) to intestinal cell membranes of the rabbit ileum and rat jejunum was investigated. Specific binding of 125I-labeled VIP could be demonstrated only on the basolateral membrane and not on the brush border membrane. This corresponded with the lack of an effect on ion transport when VIP was applied to the mucosal side of an in vitro preparation of rabbit ileum. VIP altered ion transport only when it was applied to the serosal side. The binding of 125I-VIP was specific and dependent upon incubation temperature. There was a close correlation between the potency of VIP for inhibition of 125I-VIP binding and that for increasing adenylate cyclase activity. These observations demonstrate that VIP receptors are located on the basolateral membrane.