A model for compartmentation of de novo and salvage thymidine nucleotide pools in mammalian cells.
A model for compartmentation of de novo and salvage thymidine nucleotide pools in mammalian cells.
复制标题
哺乳动物细胞中从头划分和挽救胸苷核苷酸池的模型。
DOI：
10.1073/pnas.72.9.3333
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发表时间：
1975
影响因子：
11.1
通讯作者：
R. Werner
中科院分区：
文献类型：
--
作者：
D. Kuebbing;R. Werner
当[3H]胸腺嘧啶被添加到在无胸腺嘧啶培养基中生长的HeLa细胞中时，它几乎立即以完全的特异性活性结合到DNA中，阻止任何进一步的新合成胸腺嘧啶核苷酸的结合。显然，de novo核苷酸池[Baumunk， C.N. & Friedman， D.L. (1971) Cancer Res. 31, 1930—35]和救助核苷酸池在海拉细胞中被划分。在胸腺嘧啶存在下的一代之后，细胞开始以大约相同的程度利用两个池中的核苷酸。
When [3H]thymidine is added to HeLa cells grown in thymidine-free medium it is incorporated into DNA almost immediately at full specific activity, blocking any further incorporation of de novo synthesized thymidine nucleotides. Apparently, de novo nucleotide pools [Baumunk, C.N. & Friedman, D.L. (1971) Cancer Res. 31, 1930--35] and salvage nucleotide pools are compartmentalized in HeLa cells. After one generation in the presence of thymidine, the cells begin utilizing nucleotides from both pools to about equal extent.