Differential gene expression of growth factors in young and old rat penile tissues is associated with erectile dysfunction
Differential gene expression of growth factors in young and old rat penile tissues is associated with erectile dysfunction
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DOI：
10.1038/sj.ijir.3900405
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发表时间：
1999-08-01
影响因子：
2.6
通讯作者：
Lue, TF
中科院分区：
文献类型：
--
作者：
Dahiya, R;Chui, R;Lue, TF
勃起功能障碍与年龄的分子机制尚不清楚。最近的研究表明，生长因子可能在勃起功能障碍的病因中发挥作用。本研究旨在验证大鼠阴茎组织中各种生长因子如TGF α、TGF β 1、TGF β 2、TGF β 3、IGF和NGF的基因表达随衰老而调节的假设。为此，从年轻和年老的大鼠阴茎组织中提取总RNA，并通过使用特异性寡核苷酸引物的差异逆转录-聚合酶链反应（RT-PCR）确定这些生长因子的基因表达。通过使用β-肌动蛋白作为内标来定量生长因子的mRNA水平。结果表明：（1）青年和老年大鼠阴茎组织均表达TGF-α、TGF-β 1、TGF-β 2、TGF-β 3、IGF和NGF的mRNA转录本，（2）TGF-β 1基因在老年大鼠阴茎组织中的表达明显高于青年大鼠;（3）老年大鼠阴茎组织中NGF和TGF β 3的mRNA转录水平明显低于青年大鼠;（4）青年和老年大鼠阴茎组织TGF α、TGF β 2和IGF mRNA表达无明显变化，这些结果表明，不同的基因表达的各种生长因子在年轻和老年大鼠阴茎组织可能是重要的，在了解与衰老相关的勃起功能障碍的病理生理学。
The molecular mechanisms of erectile dysfunction with aging are unclear. Recent studies have suggested that growth factors may play a role in the etiology of erectile dysfunction. This present study was designed to test the hypothesis that gene expression of various growth factors such as TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, IGF and NGF modulate with aging in rat penile tissues. For this purpose, total RNA was extracted from young and old rat penile tissues and the gene expression for these growth factors was determined by differential reverse-transcriptase-polymerase chain reaction (RT-PCR) using specific oligonucleotide primers. mRNA levels of growth factors were quantified by using p-actin as an internal standard. The results of these experiments suggest that: (1) young and old rat penile tissues expressed mRNA transcripts for TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, IGF and NGF; (2) TGF beta 1 gene expression was significantly increased in old rat penile tissues as compared to young; (3) mRNA transcripts for NGF and TGF beta 3 were significantly lower in old rat penile tissues as compared to young; and (4) TGF alpha, TGF beta 2 and IGF mRNA expression did not change in young and old rat penile tissues, These results suggest that the differential gene expression for various growth factors in young and old rat penile tissues may be important in understanding the pathophysiology of erectile dysfunction associated with aging.