Developmental Pluripotency of the Nuclei of Neurons in the Cerebral Cortex of Juvenile Mice
Developmental Pluripotency of the Nuclei of Neurons in the Cerebral Cortex of Juvenile Mice
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DOI：
10.1523/jneurosci.1591-05.2005
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发表时间：
2005-09
期刊：
影响因子：
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通讯作者：
T. Osada;N. Tamamaki;Si-young Song;N. Kakazu;Y. Yamazaki;Hatsune Makino;Ayako Sasaki;Teruyoshi Hirayama;S. Hamada;K. Nave;R. Yanagimachi;T. Yagi
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文献类型：
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作者：
T. Osada;N. Tamamaki;Si-young Song;N. Kakazu;Y. Yamazaki;Hatsune Makino;Ayako Sasaki;Teruyoshi Hirayama;S. Hamada;K. Nave;R. Yanagimachi;T. Yagi
从幼年小鼠（出生后 14-21 天）大脑皮质中绿色荧光蛋白标记的神经元中分离出的细胞核被注射到去核卵母细胞中，使其发育成囊胚。注射 2026 个神经元核后，从 76 个克隆囊胚的内细胞团建立胚胎干 (ES) 细胞系。一些 ES 细胞被单独注射到去核卵母细胞中（核移植）。其他 ES 细胞被转移到四倍体囊胚的囊胚腔中（四倍体互补）。核移植后的二细胞胚胎被转移到代孕母亲的输卵管中。 272 个核移植双细胞胚胎中有 4 个（1.5%）发育至足月，其中 2 个（0.7%）发育为可育的成体。接受 ES 细胞的 992 个四倍体囊胚中，有 19 个（1.9%）足月，其中 10 个（1.0%）发育为成体。这些发现表明，幼年小鼠大脑皮层中的一些分化神经元的细胞核保持发育多能性。
Nuclei isolated from green fluorescent protein-marked neurons in the cerebral cortex of juvenile mice (14–21 d after birth) were injected into enucleated oocytes that were allowed to develop into blastocysts. Embryonic stem (ES) cell lines were established from the inner cell mass of 76 cloned blastocysts after injecting 2026 neuronal nuclei. Some ES cells were injected individually into enucleated oocytes (nuclear transfer). Other ES cells were transferred into the blastocoeles of tetraploid blastocysts (tetraploid complementation). Two-cell embryos after nuclear transfer were transferred to the oviducts of surrogate mothers. Four (1.5%) of 272 nuclear-transferred two-cell embryos developed to term, and two (0.7%) developed into fertile adults. Nineteen (1.9%) of 992 tetraploid blastocysts receiving ES cells reached term, and 10 (1.0%) developed into adults. These findings demonstrate that some of the nuclei of differentiated neurons in the cerebral cortex of juvenile mice maintain developmental pluripotency.