High-frequency plant regeneration from leaf-disc cultures of Jatropha curcas L.:: an important biodiesel plant
High-frequency plant regeneration from leaf-disc cultures of Jatropha curcas L.:: an important biodiesel plant
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DOI：
10.1007/s11816-008-0042-y
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发表时间：
2008-04-01
影响因子：
2.4
通讯作者：
Johnson, T. Sudhakar
中科院分区：
文献类型：
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作者：
Deore, Ajay C.;Johnson, T. Sudhakar
以麻疯树叶盘为外植体，建立了一种简便、高频、可重复的不定芽诱导和植株再生方法。试管苗幼叶外植体和成熟植株在附加TDZ(2.27 mM)、6-苄氨基嘌呤(BA)(2.22 mU)和吲哚-3-丁酸(0.49 mM)的Murashige和Skoog‘s(MS)培养基上诱导出不定芽。TDZ的存在对不定芽的诱导有较大的影响，而BA在不含TDZ的条件下促进愈伤组织的诱导，而不是促进不定芽的诱导。诱导芽在附加BA(4.44 mU M)、激动素(Kn)(2.33 mU M)、吲哚-3-乙酸(IAA)(1.43 mU M)和赤霉酸(GA(3))(0.72 mU M)的MS培养基上增殖并伸长成芽。30天后，在附加IBA(0.5亩M)的MS培养基上生根。经过2个月驯化后的再生植株成功地转移到田间，没有明显的形态变化。这一协议可能用于大规模生产真实类型的植物，以及通过农杆菌/生物介导性转化生产转基因植物。
A simple, high-frequency and reproducible protocol for induction of adventitious shoot buds and plant regeneration from leaf-disc cultures of Jatropha curcas L. has been developed. Adventitious shoot buds were induced from very young leaf explants of in vitro germinated seedlings as well as mature field-grown plants cultured on Murashige and Skoog's ( MS) medium supplemented with thidiazuron (TDZ) (2.27 mu M), 6-benzylaminopurine (BA) (2.22 mu M) and indole-3-butyric acid (IBA) (0.49 mu M). The presence of TDZ in the induction medium has greater influence on the induction of adventitious shoot buds, whereas BA in the absence of TDZ promoted callus induction rather than shoot buds. Induced shoot buds were multiplied and elongated into shoots following transfer to the MS medium supplemented with BA (4.44 mu M), kinetin (Kn) (2.33 mu M), indole-3-acetic acid (IAA) (1.43 mu M), and gibberellic acid (GA(3)) (0.72 mu M). Well-developed shoots were rooted on MS medium supplemented with IBA (0.5 mu M) after 30 days. Regenerated plants after 2 months of acclimatization were successfully transferred to the field without visible morphological variation. This protocol might find use in mass production of true-to-type plants and in production of transgenic plants through Agrobacterium/biolistic-mediated transformation.