Membrane Extracts from Plant Tissues.
Membrane Extracts from Plant Tissues.
复制标题
植物组织膜提取物
DOI:
10.1007/978-1-0716-0373-4_6
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发表时间:
2020
影响因子:
--
通讯作者:
Schleiff E
中科院分区:
文献类型:
--
作者:
Bodensohn U;Ruland C;Ladig R;Schleiff E
The comparison of isolated plant cell membranous enclosures can be hampered if their extraction method differs, e.g., in regard to the utilized buffers, the tissue, or the developmental stage of the plant. Thus, for comparable results, different cellular compartments should be isolated synchronously in one procedure. Here, we devise a workflow to isolate different organelles from one tissue, which is applicable to different eudicots such asMedicagoxvariaandSolanum lycopersicum. We describe this method for the isolation of different organelles from one plant tissue for the example ofArabidopsis thaliana.All compartments are retrieved by utilizing differential centrifugation with organelle-specific parameters.
影响因子:
7.3
作者:
Hsueh, Yi-Ching;Ehmann, Christian;Schleiff, Enrico
通讯作者:
Schleiff, Enrico
影响因子:
3.9
作者:
V. Rocha;I. Ting
通讯作者:
I. Ting