Microbial diversity and anaerobic hydrocarbon degradation potential in an oil-contaminated mangrove sediment.
Microbial diversity and anaerobic hydrocarbon degradation potential in an oil-contaminated mangrove sediment.
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DOI：
10.1186/1471-2180-12-186
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发表时间：
2012-08-30
期刊：
影响因子：
4.2
通讯作者：
Rosado AS
中科院分区：
文献类型：
--
作者：
Andrade LL;Leite DC;Ferreira EM;Ferreira LQ;Paula GR;Maguire MJ;Hubert CR;Peixoto RS;Domingues RM;Rosado AS
红树林是沿海湿地，提供重要的生态系统服务，是抵御海啸、飓风和热带风暴等自然灾害的屏障。红树林拥有多种多样的生物，包括在养分循环和可得性方面发挥重要作用的微生物。由于潮汐的影响，红树林是原油从更远的地方泄漏可以积累的网站。红树林沉积物中红树林细菌多样性与石油降解之间的关系尚不清楚。从苏瑞河（巴西里约热内卢）的红树林（有石油污染史）中，从0-5、15-20和35-40厘米深度间隔采集了红树林沉积物样本。bamA、dsr和16 S rRNA编码片段基因的DGGE指纹图谱以及使用dsr和16 S rRNA基因片段的qPCR分析显示了沉积物深度的差异。细菌16 S rRNA基因多样性分析显示，随着深度的变化。DGGE分析结果表明，在5 ~ 15 cm深度范围内，厌氧烃类降解菌群分布也发生了变化，且在两个深度范围内的分布基本一致，表明在15 cm以下的红树林沉积物中，厌氧烃类降解菌群分布较为均匀。qPCR分析显示，沉积物深度不同，顶层（0-5厘米）的细菌丰度一般高于两个较深沉积物层（15-20厘米和35-40厘米），这两个较深沉积物层彼此相似。
Mangrove forests are coastal wetlands that provide vital ecosystem services and serve as barriers against natural disasters like tsunamis, hurricanes and tropical storms. Mangroves harbour a large diversity of organisms, including microorganisms with important roles in nutrient cycling and availability. Due to tidal influence, mangroves are sites where crude oil from spills farther away can accumulate. The relationship between mangrove bacterial diversity and oil degradation in mangrove sediments remains poorly understood. Mangrove sediment was sampled from 0–5, 15–20 and 35–40 cm depth intervals from the Suruí River mangrove (Rio de Janeiro, Brazil), which has a history of oil contamination. DGGE fingerprinting for bamA, dsr and 16S rRNA encoding fragment genes, and qPCR analysis using dsr and 16S rRNA gene fragment revealed differences with sediment depth. Analysis of bacterial 16S rRNA gene diversity revealed changes with depth. DGGE for bamA and dsr genes shows that the anaerobic hydrocarbon-degrading community profile also changed between 5 and 15 cm depth, and is similar in the two deeper sediments, indicating that below 15 cm the anaerobic hydrocarbon-degrading community appears to be well established and homogeneous in this mangrove sediment. qPCR analysis revealed differences with sediment depth, with general bacterial abundance in the top layer (0–5 cm) being greater than in both deeper sediment layers (15–20 and 35–40 cm), which were similar to each other.
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影响因子：
3
作者：
Grossi, Vincent;Cravo-Laureau, Cristiana;Hirschler-Rea, Agnes
通讯作者：
Hirschler-Rea, Agnes
影响因子：
5.1
作者：
Leloup, Julie;Loy, Alexander;Jorgensen, Bo Barker
通讯作者：
Jorgensen, Bo Barker
影响因子：
56.9
作者：
Habicht, KS;Gade, M;Canfield, DE
通讯作者：
Canfield, DE
DOI：
10.1016/s1353-2561(00)00056-6
发表时间：
2000-01-01
期刊：
SPILL SCIENCE & TECHNOLOGY BULLETIN
影响因子：
--
作者：
Michel, J
通讯作者：
Michel, J
影响因子：
11.4
作者：
Beller, HR;Kane, SR;Alvarez, PJJ
通讯作者：
Alvarez, PJJ