Preparation and characterization of nanoclay-hydrogel composite support-bath for bioprinting of complex structures
Preparation and characterization of nanoclay-hydrogel composite support-bath for bioprinting of complex structures
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DOI：
10.1038/s41598-020-61606-x
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发表时间：
2020-03-24
影响因子：
4.6
通讯作者：
Koc, Bahattin
中科院分区：
文献类型：
--
作者：
Afghah, Ferdows;Altunbek, Mine;Koc, Bahattin
最近，在牺牲支撑浴中对细胞水凝胶进行三维生物打印成为制造复杂生物结构的一种潜在解决方案。支撑浴的物理性质强烈地影响生物印刷过程和所制造的构件的结果。在这项研究中，我们报道了一种含钙离子的Pluronic-纳米粘土复合支撑浴作为交联剂应用于细胞负载藻酸盐水凝胶的生物印花。通过调整流变性，制备了具有快速自恢复行为的剪切变稀复合支撑浴，实现了复杂大尺寸结构的连续印花。在不影响其形状保真度的情况下，可轻松高效地从支撑浴中获得印刷结构。此外，结果表明，支持浴辅助生物打印过程不影响水凝胶包裹的细胞的活力。这项研究表明，Pluronic纳米粘土支撑浴可用于血管和其他组织工程应用的复杂细胞结构的生物打印。
Three-dimensional bioprinting of cell-laden hydrogels in a sacrificial support-bath has recently emerged as a potential solution for fabricating complex biological structures. Physical properties of the support-bath strongly influence the bioprinting process and the outcome of the fabricated constructs. In this study, we reported the application of a composite Pluronic-nanoclay support-bath including calcium ions as the crosslinking agent for bioprinting of cell-laden alginate-based hydrogels. By tuning the rheological properties, a shear-thinning composite support-bath with fast self-recovery behavior was yielded, which allowed continuous printing of complex and large-scale structures. The printed structures were easily and efficiently harvested from the support-bath without disturbing their shape fidelity. Moreover, the results showed that support-bath assisted bioprinting process did not influence the viability of cells encapsulated within hydrogel. This study demonstrates that Pluronic-nanoclay support-bath can be utilized for bioprinting of complex, cell-laden constructs for vascular and other tissue engineering applications.